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当前位置: 首页 > 文章 > 肉鸡生产链中肠炎沙门氏菌耐药性分析及ERIC-PCR分型 食品科学 2016,37 (3) 120-124
Position: Home > Articles > Resistance and ERIC-PCR Genotyping of Salmonella enteritidis in Broiler Production Chain FOOD SCIENCE 2016,37 (3) 120-124

肉鸡生产链中肠炎沙门氏菌耐药性分析及ERIC-PCR分型

作  者：
李楠;赵思俊;王娟;赵建梅;李玉清;黄秀梅;王君玮;丁宜宝;刘焕奇
单  位：
青岛农业大学动物科技学院;中国动物卫生与流行病学中心;中国兽医药品监察所
关键词：
肠炎沙门氏菌;肉鸡;耐药性;肠杆菌基因间重复共有序列-聚合酶链式反应
摘  要：
目的:了解肉鸡生产链中肠炎沙门氏菌耐药性情况以及各生产环节菌株间亲缘关系,为临床用药及菌株追踪溯源提供依据。方法:采用微量肉汤稀释法对171株肠炎沙门氏菌进行13种抗菌药物药敏实验;采用肠杆菌基因间重复共有序列-聚合酶链式反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERIC-PCR)法对33株不同生产环节耐药菌株进行分子分型;采用SPSS 20.0软件、Gel-Pro Analyer 4.0和NTSYS pc 2.1软件进行分析。结果:171株肠炎沙门氏菌对13种药物耐药情况不同,其中对氨苄西林耐药情况最严重,耐药率高达90.06%,对恩诺沙星、氧氟沙星最为敏感,耐药率均仅为5.26%;不同环节间耐药性差异极显著(P<0.01);多重耐药率高达95.32%,共有26种耐药谱型。不同环节的33株肠炎沙门氏菌分为4种(Ⅰ~Ⅳ)基因型,遗传相似性在66%~100%,Ⅰ型为优势基因型;基因型相同的菌株耐药谱不一定相同,反之,耐药谱相同的菌株基因型不一定相同。结论:肉鸡生产链条中沙门氏菌对多种抗菌药物产生耐药性,且耐药谱种类繁多;沙门氏菌能够沿着生产链进行水平传播,肉鸡场环节菌株基因型相对复杂,菌株基因型与耐药表型之间无明显相关性。
译  名：
Resistance and ERIC-PCR Genotyping of Salmonella enteritidis in Broiler Production Chain
作  者：
LI Nan;ZHAO Sijun;WANG Juan;ZHAO Jianmei;LI Yuqing;HUANG Xiumei;WANG Junwei;DING Yibao;LIU Huanqi;QU Zhina;China Animal Health and Epidemiology Center;School of Animal Science and Technology, Qingdao Agricultural University;China Institute of Veterinary Drug Control;
关键词：
Salmonella enteritidis;;broiler;;antimicrobial resistance;;enterobacterial repetitive intergenic consensuspolymerase chain reaction
摘  要：
Objective: To investigate the antimicrobial resistance and genetic relationship of Salmonella enteritidis in the production chain of broiler, and to provide the basis for clinical treatment and strain traceability. Methods: The susceptibility of 171 Salmonella enteritidis to 13 antimicrobial agents was analyzed by micro-dilution method. The molecular types of 33 strains from different production links associated with resistance were analyzed by ERIC-PCR. The data were analyzed by SPSS 20.0 software, Gel-Pro analyzer 4.0 and NTSYS pc 2.1 software. Results: The resistance degrees of 171 Salmonella enteritidis to 13 antimicrobial agents were different. The resistance rate of these strains to ampicillin was the highest and up to 90.06%, and the resistance rate to ofl oxacin was the most sensitive and only 5.26%. The resistance rates of different links were signifi cantly different(P < 0.01). The multidrug resistance rate was 95.32%, and there were 26 kinds of anti-biograms. Totally 33 Salmonella enteritidis were divided into 4(I–IV) different genotypes, and the genetic similarity was 66%–100%, and type I was the dominant genotype. Anti-biograms between the same genotype were also different; conversely, the genotypes between the same antibiogram were different. Conclusion: The Salmonella enteritidis in broiler production chain could generate resistance to many antimicrobial agents, generating many kinds of antibiograms. The Salmonella enteritidis could be spread horizontally along the production chain. The genotypes of broiler farm were relatively complex. There was no signifi cant correlation between genotype and resistant phenotype.

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