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Position: Home > Articles > Screening,identification and phylogenetic analysis of marine microorganisms producing extracellular esterase Progress in Fishery Sciences 2009,30 (3) 70-75

产酯酶海洋微生物的筛选、鉴定及系统发育分析

作  者:
郑鸿飞;孙谧;王跃军;郝建华;高强
单  位:
中国水产科学研究院黄海水产研究所海洋产物资源与酶工程实验室
关键词:
海洋微生物;酯酶;16S rDNA;系统发育学分析
摘  要:
通过以三丁酸甘油酯和α-乙酸萘酯为底物,建立了一种快速、准确的酯酶产生菌的筛选方法,并从样品中分离到1株产酯酶活力和稳定性较高的菌株EB-1,对其进行了形态学、生理生化特征鉴定和16S rDNA序列分析。结果表明,该菌株与Bacillus subtilis subsp.subtilis模式菌株的同源性高达99.79%;又根据其产黑色素这一特性,最终将其确定为Bacillus subtilis var.niger,其16S rDNA序列在GenBank的注册号为EU016526。
译  名:
Screening,identification and phylogenetic analysis of marine microorganisms producing extracellular esterase
作  者:
ZHENG Hong-fei 1, 2 SUM Mi 1 WANG Yue-jun 1 HAO Jian-hua 1 GAO Qiang 1(1 Laboratory of Marine Products and Enzyme Engineering,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071)(2 College of Food Science,Shanghai Ocean University,201306)
关键词:
Marine microorganism Esterase α-Naphthyl acetate Phylogenetic analysis
摘  要:
With α-naphthyl acetate and tributyrin as substrate,a precise and rapid screening method was developed.One stable strain-EB-1,which was isolated from related samples,showed the highest esterase-producing ability among the 281 strains.Through study on its morphological,physiological and biochemical characteristics as well as 16S rDNA sequence homology comparison,strain-EB-1 was identified as Bacillus subtilis var.niger,which was almost the closest relative to the standard strain with 99.79% similarity in sequence under the phylogenetic tree.The 16S rDNA sequence of strain-EB-1 has been deposited in GenBank,registration number was EU016526.

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