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当前位置: 首页 > 文章 > 维氏气单胞菌拮抗菌株J2-2的筛选、鉴定与效果评价 淡水渔业 2024,54 (1) 36-44
Position: Home > Articles > Isolation,identification and effect evaluation of antagonistic strain J2-2 from Aeromonas veronii Freshwater Fisheries 2024,54 (1) 36-44

维氏气单胞菌拮抗菌株J2-2的筛选、鉴定与效果评价

作  者：
程心怡;孙正祥;苏应兵;周燚
单  位：
长江大学动物科学学院;长江大学农学院
关键词：
解淀粉芽孢杆菌(Bacillus amyloliquefaciens);维氏气单胞菌(Aeromonas veronii);安全性评价;LC-MS
摘  要：
为筛选对维氏气单胞菌(Aeromonas veronii)有潜在抑菌作用的菌株,本研究从湖北公安县泥鳅养殖池塘底泥中分离一株细菌J2-2,对其特性进行了研究,并对抑菌活性物质进行了初步分析.结果显示:菌株J2-2 为解淀粉芽孢杆菌(Bacillus amyloliquefaciens),具有抑菌广谱性,对米尔伊丽莎白菌(Elizabethella milieri)、嗜水气单胞菌(A.hydrophila)等6 种水产病原菌的抑菌直径在9.6~29.5 mm.菌株J2-2 的抑菌物质具有较好的酸碱稳定性、热稳定性和蛋白酶K处理的稳定性.电镜结果显示菌株J2-2 的发酵产物对维氏气单胞菌的细胞膜结构具有破坏作用.通过LC-MC分析该拮抗细菌可产生至少 15 种不同的脂肽类化合物如杆菌霉素等.药敏试验结果显示,菌株J2-2 对氨苄西林、青霉素具有耐药性,对克林霉素中度敏感,对大多数抗生素高度敏感.
译  名：
Isolation,identification and effect evaluation of antagonistic strain J2-2 from Aeromonas veronii
关键词：
Bacillus amyloliquefaciens%Aeromonas veronii%safety evaluation%LC-MS
摘  要：
This study isolated a strain J2-2 from the bottom mud of loach aquaculture pond in Gongan County,Hubei Province,to screen the bacteria with potential antibacterial effect on Aeromonas veronii.Meanwhile,its char-acteristics were studied and the antibacterial active substances were preliminarily analyzed.The results showed that the strain J2-2 is Bacillus amyloliquefaciens,which has a broad bacteriostatic spectrum.The inhibition diameters of 6 aquatic pathogens,such as Elizabethella milieri and A.hydrophila,ranged from 9.6 to 29.5mm.The bacterio-static substances of strain J2-2 were insensitive to high temperature,acid,acid-base,and protease.The results of electron microscopy showed that the fermentation product of strain J2-2 had destructive effect on the cell mem-brane structure of A.veronii.Through LC-MC analysis,the antagonistic bacteria could produce at least 15 li-popeptide compounds such as Bacillomycin D,etc.The susceptibility test results showed that strain J2-2 was on-ly resistant to ampicillin and penicillin,moderately susceptible to clindamycin and highly susceptible to most anti-biotics.

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