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Position: Home > Articles > Effects of CuSO_4 Stress on Seed Germination and Seedlings Physiological Indicators of Cercis chinensis Hubei Agricultural Sciences 2012,51 (18) 4047-4051

重金属Cu~(2+)对紫荆种子萌发和幼苗生理的影响

作  者:
周建;马敏
单  位:
河南科技学院园艺园林学院
关键词:
紫荆;Cu2+胁迫;种子;幼苗;生理指标
摘  要:
为研究重金属Cu2+对紫荆幼苗生长影响的生理机制,试验利用CuSO4溶液的浓度梯度对紫荆种子进行了胁迫处理,分别测定紫荆种子的发芽率、发芽指数、活力指数等发芽指标和幼苗叶片的丙二醛、可溶性糖、脯氨酸含量及过氧化物酶活性、细胞膜透性等生理指标。结果表明,紫荆种子抗重金属Cu2+胁迫的能力比较强,在致死状态下,CuSO4溶液的浓度为183.40mg/L;在半致死状态下,CuSO4溶液的浓度为104.22 mg/L。随着CuSO4溶液浓度的增加,紫荆种子的发芽率、发芽指数、活力指数逐步下降,幼苗叶片的细胞膜透性和丙二醛、可溶性糖、脯氨酸含量均有不同程度地增加,而幼苗叶片的过氧化物酶活性则呈现出先增加后递减的变化趋势。
译  名:
Effects of CuSO_4 Stress on Seed Germination and Seedlings Physiological Indicators of Cercis chinensis
作  者:
ZHOU Jian,MA Min(School of Horticulture and Landscape Architecture,Henan Institute of Science and Technology,Xinxiang 453003,Henan,China)
关键词:
Cercis chinensis Bunge;Cu2+ stress;seeds;seedlings;physiological indicators
摘  要:
To study the physiological mechanisms of Cu2+ affecting seedling growth of Cercis chinensis Bunge,seed of C.chinensis was stressed by CuSO4 solution;and germination rate,germination index,vigor index of seeds and content of malondialdehyde(MDA),soluble sugar,Proline(Pro) and peroxidase(POD) activity and cell membrane permeability in seedling was determined.The results showed that C.chinensis seeds had strong tolerance against heavy metal Cu2+ stress as LC100 and LC50 were 183.40 and 104.22 mg/L respectively.With the increase of CuSO4 concentration,germination rate,germination index,vigor index of C.chinensis seed decreased gradually;cell membrane permeability and MDA,soluble sugar,Pro content in seedling increased at different degree;while POD activity showed a decrease trend after increased firstly.

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