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当前位置: 首页 > 文章 > 夏橙果实离层中伤害诱导基因的克隆与表达分析 果树学报 2010,27 (6) 10-16
Position: Home > Articles > Cloning of wound-induced protein genes from Citrus sinensis cv. Valencia and analysis of their expression patterns in fruit calyx abscission zone Journal of Fruit Science 2010,27 (6) 10-16

夏橙果实离层中伤害诱导基因的克隆与表达分析

作  者：
杨雪莲;张凌云;王志卫;黄勇;闫树堂;钟广炎
单  位：
西南大学;贵州大学;中国农业科学院柑桔研究所
关键词：
夏橙;伤害诱导蛋白;基因家族;克隆;乙烯诱导
摘  要：
从乙烯处理的伏令夏橙果实离层中克隆出4个伤害诱导基因(CsWIP1、CsWIP2、CsWIP3、CsWIP4)。通过RT-PCR、RACE等克隆技术分别获得了CsWIP1、CsWIP2、CsWIP3和CsWIP4的全长cDNA。测序结果表明CsWIP1、CsWIP2、CsWIP3和CsWIP4全长cDNA分别为588、549、412和629bp,开放阅读框分别编码98、95、83和88个氨基酸,结构模拟显示这些蛋白在C-端和N-端各有一个α-螺旋,中间是一个富丝氨酸域。与其他植物伤害诱导蛋白序列比对结果显示,CsWIP家族蛋白分为2类,CsWIP1、CsWIP2和CsWIP3与玉米WIP家族中NP_001148450.1、NP_001151919.1、NP_001151459.1、NP_001147041.1和拟南芥NP_192765.1、NP_849355.1、NP_179023.1聚成一类;CsWIP4与葡萄的AY159560.1聚成一类。通过实时定量PCR检测这些基因在乙烯处理下果实离层中的表达情况,结果显示CsWIP1和CsWIP2受乙烯强烈诱导,CsWIP3和CsWIP4几乎不受乙烯调控。
译  名：
Cloning of wound-induced protein genes from Citrus sinensis cv. Valencia and analysis of their expression patterns in fruit calyx abscission zone
作  者：
YANG Xue-lian1,3,ZHANG Ling-yun1,WANG Zhi-wei1,HUANG Yong1,YAN Shu-tang1,2,ZHONG Guang-yan1,2 (1Southwest University,Beibei,Chongqing 400715 China;2Citrus Research Institute,Chinese Academy of Agricultural Sciences,Beibei,Chongqing 400712 China;3Guizhou University,Guiyang,Guizhou 550025 China)
关键词：
Citrus sinensis cv. Valencia;Wound-induced protein (WIP);Gene family;Cloning;Ethylene induction
摘  要：
Four wound-induced genes,CsWIP1,CsWIP2,CsWIP3 and CsWIP4,were cloned from ethylene-treated abscission zones ( AZ-C ) of citrus (Citrus sinensis cv.Olinda valencia ).Their full-length cDNAs were obtained by using RT-PCR and RACE.The full-length cDNAs of CsWIP1,CsWIP2,CsWIP3 and CsWIP4 were 588,549,412 and 629 bp and the corresponding ORFs would encode 98,95,83 and 88 amino-acid residues,respectively.Structural simulations showed that the deduced proteins possessed two -helices located separately at Nand C-terminals and were rich in serine residues in the middle.Alignments with other plant wound-induced proteins showed that the citrus wound-induced proteins could be grouped into two classes: CsWIP1,CsWIP2 and CsWIP3 were clustered together with corn NP_001148450.1,NP_001151919.1,NP_001151459.1,NP_001147041.1 and arabidopsis NP_192765.1,NP_849355.1,NP_179023.1 while CsWIP4 was clustered together with grape AY159560.1.Quantitative real time PCR was used to study the expression patterns of these genes.In AZ-C,the expression of CsWIP1 and CsWIP2 was strongly induced by ethylene,whereas the expression of CsWIP3 and CsWIP4 was not significantly modulated by ethylene.

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