中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 应用均匀设计法优化两种野生药用及观赏杜鹃花的高效快繁体系 安徽农业科学 2009,37 (28) 3566-3568
Position: Home > Articles > Optimization on the High Efficient Propagation System of Two Wild Medicinal and Ornamental Azaleas by Uniform Design Journal of Anhui Agricultural Sciences 2009,37 (28) 3566-3568

应用均匀设计法优化两种野生药用及观赏杜鹃花的高效快繁体系

作  者：
冯颖;鞠月秋;顾地周
单  位：
通化市第一中学;通化师范学院生物系
关键词：
迎红杜鹃;兴安杜鹃;均匀设计;组织培养;快速繁殖
摘  要：
[目的]筛选迎红杜鹃和兴安杜鹃的最佳生长培养基,建立其高效快繁体系。[方法]以迎红杜鹃和兴安杜鹃的嫩茎段为外植体,以改良MS为基本培养基,附加不同浓度的IAA、IBA、NAA和GA_3,应用均匀设计法,选用U_(10)(10~4)均匀表,考察了4种激素不同浓度配比对2种杜鹃嫩茎腋芽生长伸长及茎段生根诱导率的影响,并以2种杜鹃再生植株的茎节为材料,以节培法进行快繁,建立高效快繁体系。[结果]最适合迎红杜鹃嫩茎腋芽生长伸长和嫩茎段生根的培养基为:改良MS+IAA 0.10 mg/L+GA_31.20 mg/L,再生率97%;最适合兴安杜鹃生长的培养基为:改良MS+IBA0.10 mg/L+GA_31.20 mg/L,再生率98%。对2种杜鹃再生植株的快繁表明,在35 d的一个培养周期内,增殖倍数达60以上。[结论]成功建立了迎红杜鹃和兴安杜鹃的高效离体快繁体系。
译  名：
Optimization on the High Efficient Propagation System of Two Wild Medicinal and Ornamental Azaleas by Uniform Design
作  者：
FENG Ying et al(Department of Biology,Tonghua Normal University,Tonghua,Jilin 134002)
关键词：
Rhododendron mucronulatum Turcz.;;Rhododendron dauriumL.;;Uniform design;;Tissue culture;;Rapid propagation
摘  要：
[Objective]The study aimed to screen the optimum growing medium for Rhododendron mucronulatum Turcz.and Rhododendron daurium L.and establish the high efficient micropropagation system.[Method]With Young stems of R.mucronulatum and R.daurium as the explants,the modified MS medium was taken as the basic medium added by IAA,IBA,NAA and GA_3 with different concn.to investigate the effect of 4 kinds of hormones with different concn.matching on the axillary bud growing of young stems and stem rooting of 2 kinds of azaleas through selecting U_(10)(10~4 ) uniform table by uniform design method.The stem-nods of the regenerated plants were used as materials to make for micropropagation by nod culture metod so as to set up the high efficient micropropagation system.[Result]The optimum medium for the axillary bud growing and rooting of young stems for R.mucronulatum was follows:the modified MS + IAA 0.10 mg/L + GA_31.20 mg/L,with regeneration rate of 97%,and that for R.daurium was follows:the modified MS + IBA 0.10 mg/L + GA_3 1.20 mg/L,with a regeneration rate of 98%.The micropropagation made on the regenerated plants of 2 kinds of azaleas showed that 60-fold proliferation rate was achieved within a culture cycle of 35 d.[Conclusion]High efficient micropropagation systems of A.mucronulatum and R.daurium were successfully established.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46