中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 桦褐孔菌原生质体制备与再生 食品科学 2011,32 (13) 161-164
Position: Home > Articles > Preparation and Regeneration of Inonotus obliquus Protoplasts FOOD SCIENCE 2011,32 (13) 161-164

桦褐孔菌原生质体制备与再生

作  者：
孙勇;曹小迎;陈永强;缪倩;蒋继宏
单  位：
徐州师范大学江苏省药用植物生物技术重点实验室
关键词：
桦褐孔菌;原生质体;正交试验
摘  要：
研究桦褐孔菌原生质体制备与再生条件。采用正交试验法确定复合酶比例,用单因素试验测定不同等渗液、酶解时间、桦褐孔菌菌龄、酶解温度、再生培养基对桦褐孔菌原生质体制备与再生的影响。结果表明:混合酶(纤维素酶5mg/mL、蜗牛酶15mg/mL、溶壁酶15mg/mL、崩溃酶15mg/mL)、菌龄为7d的菌丝,采用0.6mol/LMgSO4为渗透压稳定液,酶解温度35℃,酶解时间5.5h,有利于原生质体的制备。就原生质体再生而言,酶解时间为3.5h制得的原生质体,以MgSO4作为渗透压稳定液,以完全再生培养基(CYM)和麦芽酵母葡萄糖再生培养基(GYM)有利于原生质体再生,再生率最高为0.13%。
译  名：
Preparation and Regeneration of Inonotus obliquus Protoplasts
作  者：
SUN Yong,CAO Xiao-ying,CHEN Yong-qiang,LIAO Qian,JIANG Ji-hong(Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province,Xuzhou Normal University,Xuzhou 221116,China)
关键词：
Inonotus obliquus;protoplast;orthogonal test
摘  要：
The conditions for protoplast preparation and regeneration from the mycelia of Inonotus obliquus were investigated.The optimal amounts of four enzymes were explored by orthogonal array design and other conditions were studied by single factor method.The results showed that the mycelium harvesting after 7-day culture,adding 5 mg/mL cellulase,15 mg/mL snailase,15 mg/mL lywallzyme and 15 mg/mL drislease,using 0.6 mol/L MgSO4 as the osmotic stabilizer,digestion at 35 ℃ for 5.5 h were found to be best for the preparation of protoplasts.The optimal conditions for protoplast regeneration of Inonotus obliquus were CYM or GYM as the medium,0.6 mol/L mannitol as the osmotic stabilizer using and digestion time of 3.5 h.No significant difference in protoplast regeneration rate was observed between CYM and GYM media.The highest protoplast regeneration rate was 0.13%.

相似文章

导出引用
计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46