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Position: Home > Articles > Extraction and antioxidant activity of avenanthramides from oat bran Transactions of the Chinese Society of Agricultural Engineering 2008,24 (5) 265-269

燕麦生物碱的提取及其抗氧化活性研究

作  者:
任祎;任贵兴;马挺军;平华;牛西午
单  位:
山西省农业科学院;山西农业大学农学院;中国农业科学院作物科学研究所
关键词:
燕麦麸皮;生物碱;提取;抗氧化活性
摘  要:
采用正交试验设计对麸皮中燕麦生物碱(AV)的提取条件进行优化,提取物经大孔吸附树脂纯化,并利用活性氧和DPPH自由基的清除率研究其抗氧化活性。结果表明:最佳提取参数为温度60℃,提取时间2h,溶剂为乙醇/水/冰醋酸(80/19.9/0.1),料液比1︰8,此条件下得率为5.29%,经树脂纯化后粗提物中生物碱纯度达19.2%,且纯化后的燕麦生物碱表现出较强的清除OH.,O2-.和有机自由基DPPH的体外抗氧化活性,其清除能力分别是α-生育酚的79.4%、82.2%和78.0%。
译  名:
Extraction and antioxidant activity of avenanthramides from oat bran
作  者:
Ren Yi1,2,Ren Guixing2,Ma Tingjun2,Ping Hua2,Niu Xiwu3(1.Shanxi Agricultural University,Taigu 030801,China;2.The Chinese Academy of Agricultural Sciences,Institute of Crop Science,Beijing100081,China;3.Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)
关键词:
oat bran;avenanthramides;extraction;antioxidant activity
摘  要:
The technology for extraction avenanthramides from oat bran was optimized by Orthogonal design,and purified by macroporous resin;Meanwhile,the vitro antioxidant activity were studied.Results show that the optimal parameters for extraction are ethanol / water / acetic acid 80/19.9/0.1,temperature 60℃,solid-liquid ratio of 1︰8,the extraction time is 2 h.Under such conditions,the yield of avenanthramides is 5.29%,the content of avenanthramides can be increased up to 19.2% after being purified by AB-8 macroporous resin.It also show that the purified avenanthramides extract has strong capacity of scavenging OH.,O2-.and DPPH free radical,the scavenging capacity are 79.4%,82.2% and 78.0% as large as that of α-tocopherol respectively.

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