作 者：
闫峻;李泽青;王文杰;刘通;马衍旋;李宁;马墉;郑梓;穆淑琴
关键词：
L-茶氨酸;断奶仔猪;氧化应激;生长性能;体液免疫
摘 要：
本研究探讨了L-茶氨酸对氧化应激断奶仔猪生长性能、抗氧化能力及免疫功能的影响。试验采用2×2二因素设计,160头断奶仔猪(7.53kg±0.51kg)随机分为4个处理组:1组(日粮中不添加L-茶氨酸,腹腔注射5mL生理盐水);2组(日粮中不添加L-茶氨酸,腹腔注射8 mg/kg体重敌快死);3组(日粮中添加1 000mg/kg L-茶氨酸,腹腔注射5mL生理盐水);4组(日粮中添加1 000mg/kg L-茶氨酸,腹腔注射8mg/kg体重敌快死)。预试期7d,正试期28d。结果显示:(1)与1组相比,2组仔猪平均日增重(ADG)和平均日采食量(ADFI)显著降低,而料重比显著升高(P<0.05);与2组相比,4组仔猪ADG显著增加(P<0.05)。(2)与1组相比,2组仔猪血清丙二醛(MDA)含量显著增加,而总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GSH-Px)活性显著下降(P<0.05),3组仔猪血清MDA显著降低,而T-AOC显著增加(P<0.05);与2组相比,4组仔猪血清MDA含量显著降低,而T-AOC和GSH-Px活性显著上升(P<0.05)。(3)与1组相比,2组仔猪血清IgA、IgG、IL-2、IL-4水平显著增加,C3和C4水平显著下降(P<0.05),3组仔猪血清IgA、IgG和IL-4水平显著降低,而C3和C4水平显著增加(P<0.05);与2组相比,4组仔猪血清IgA、IgG、IL-2、IL-4水平显著降低,而血清C3水平显著增加(P<0.05)。结果表明,注射敌快死可引发仔猪氧化应激,造成生长性能下降、体液免疫能力下降;日粮中添加1 000mg/kg L-茶氨酸可缓解仔猪氧化损伤,提高氧化应激仔猪的生长性能、抗氧化能力和体液免疫能力。
译 名：
Effects of L-theanine on Growth Performance and Blood Biochemical Indicators of Oxidative Stress Weaned Piglets
作 者：
YAN Jun;LI Zeqing;WANG Wenjie;LIU Tong;MA Yanxuan;LI Ning;MA Yong;ZHENG Zi;MU Shuqin;Tianjin Institute of Animal Science and Veterinary,Tianjin Academy of Agricultural Science;College of Chemical Engineering,Tianjin University;Tianjin Animal Husbandry and Veterinary Station;College of Animal Science and Technology,Northwest A&F University;
单 位：
YAN Jun%LI Zeqing%WANG Wenjie%LIU Tong%MA Yanxuan%LI Ning%MA Yong%ZHENG Zi%MU Shuqin%Tianjin Institute of Animal Science and Veterinary,Tianjin Academy of Agricultural Science%College of Chemical Engineering,Tianjin University%Tianjin Animal Husbandry and Veterinary Station%College of Animal Science and Technology,Northwest A&F University
关键词：
L-theanine;;weaned piglet;;oxidative stress;;growth performance;;humoral immunity
摘 要：
To explore the effects of L-theanine on growth performance,antioxidant and immune response of oxidative stress weaned pigs,a two-factor(2×2)experimental design was employed.160 weaned pigs(7.53 kg±0.51 kg)were divided into 4 treatment groups:Treatment 1(withoutL-theanine,intraperitoneal injection of 5 mL normal saline),treatment 2(without L-theanine,intraperitoneal injection of 8 mg/(kg·BW)diquat),treatment 3(diet added 1 000 mg/kg L-theanine,intraperitoneal injection of 5 mL normal saline)and treatment 4(diet added 1 000 mg/kg Ltheanine,intraperitoneal injection of 8 mg/(kg·BW)diquat).7 dfor preliminary feeding and 28 d for formal feeding.The results showed that:(1) Compared to treatment 1,the average daily gain(ADG)and average daily feed intake(ADFI)of weaned pigs in treatment 2 decreased significantly(P<0.05),while F/G increased significantly(P<0.05);Compared to treatment 2,L-theanine significantly increased ADG and ADFI in treatment 4(P<0.05).(2)Compared to treatment 1,diquat significantly increased serum malondialdehyde(MDA)content,while the total antioxidant capacity(T-AOC)and the activity of glutathione peroxidase(GSH-Px)significantly decreased in treatment 2(P<0.05),serum MDA content in treatment 3 significantly increased while the activity of GSH-Px significantly tended down(P<0.05);Compared to treatment 2,L-theanine significantly decreased serum MDA content while increased the activity of T-AOC and GSH-Px in treatment 4(P<0.05).(3)Compared to treatment 1,the levels of serum IgA,IgG and IL-2,IL-4 significantly increased,while C3 and C4 significantly decreased(P<0.05)in treatment 2,and the levels of serum IgA,IgG and IL-4 were significantly decreased,while the concentration of C3 and C4 significantly increased in treatment 3(P<0.05);Compared to treatment 2,the levels of serum IgA,IgG and IL-2,IL-4 significantly decreased,while C3 significantly increased in treatment 4(P<0.05).In conclusion,injection of diquat could cause oxidative stress in piglets,resulting decrease of growth performance and humoral immunity;Diets added 1 000 mg/kg L-theanine could relieve oxidative damage,improve the growth performance,the capacity of antioxidation and humoral immunity in oxidative stressed piglets.
