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Position: Home > Articles > Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck Fujian Journal of Agricultural Sciences 2014 (11) 1066-1069

樱桃谷种鸭鸭1型甲肝病毒的分离鉴定及其VP1基因的序列分析

作  者:
陈红梅;陈珍;程龙飞;傅光华;施少华;万春和;傅秋玲;黄瑜
单  位:
福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心
关键词:
樱桃谷种鸭;鸭1型甲肝病毒;VP1基因
摘  要:
从某樱桃谷种鸭场病死樱桃谷种鸭的肝脏中分离到1株病毒(命名为HeN1403),该病毒能在79h内致死10日龄番鸭胚,对8日龄雏番鸭的致死率为33.3%。应用鸭1型甲肝病毒特异性引物对HeN1403分离株进行RT-PCR检测,可扩增到约200bp的目的条带,并对分离株的VP1基因进行扩增,得到714bp的基因片段。通过软件对分离株VP1基因序列与GenBank上发表的12株不同基因型鸭1型甲肝病毒的VP1基因核苷酸序列的同源性进行比对和遗传进化分析,确定该分离株为鸭1型甲肝病毒。
译  名:
Identification and Sequence Analysis of Duck Hepatitis Virus A Type 1 Isolated from Cherry Valley Breeding Duck
作  者:
CHEN Hong-mei;CHEN Zhen;CHENG Long-fei;FU Guang-hua;SHI Shao-hua;WAN Chun-he;FU Qiu-ling;HUANG Yu;Animal Husbandry and Veterinary Institute,Fujian Academy of Agricultural Sciences/Fujian Animal Disease Control Technology Development Center;
关键词:
Cherry Valley Breeding duck;;duck hepatitis virus a type 1;;VP1gene
摘  要:
A virus strain,named HeN1403,was isolated from the liver of dead Cherry Valley Breeding ducks.The virus killed 10 days Muscovy duck embryo within 79 hours and showed 33.3% of mortality rate to eight-day-old Muscovy ducklings.HeN1403 was characterized by specific RT-PCR primers which target to duck hepatitis virus A type 1,and it turned out to be a 200 bp fragment.A 714-bp fragment gene was obtained by using a set of primers aimed at VP1 ORF gene.Genetic analysis showed that the isolated virus was one of pancreatitis-type duck hepatitis virus A type 1.

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