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Position: Home > Articles > Determination of α-tocopherol content in millet by HPLC method Acta Agriculturae Shanghai 2016,32 (2) 150-152

HPLC法测定小米α-生育酚含量

作  者:
王凌云;马海莲;宋晋辉;瓮巧云;袁进成;刘颖慧;赵治海
单  位:
河北北方学院;张家口市农业科学院
关键词:
α-生育酚;小米;HPLC;含量
摘  要:
建立了以C18反相柱为分析柱,以甲醇-水为流动相的反相液相色谱法,采用1 mL/min的流速在检测波长为280 nm处对小米中α-生育酚的含量进行测定。结果表明:α-生育酚在质量浓度为0.5—100μg/mL范围内线性良好,R~2=0.9993,回收率83%—99%。在选定的5种小米中,α-生育酚含量各异,可能与品种有关。该方法操作简便,结果准确,可用于小米α-生育酚的含量测定。
译  名:
Determination of α-tocopherol content in millet by HPLC method
作  者:
WANG Ling-yun;MA Hai-lian;SONG Jin-hui;WENG Qiao-yun;YUAN Jin-cheng;LIU Ying-hui;ZHAO Zhi-hai;Hebei North University;Zhangjiakou Academy of Agricultural Sciences;
关键词:
α-tocopherol;;Millet;;HPLC;;Content
摘  要:
A high performance liquid chromatographic(HPLC) method with C18 reversed phase column as the analytical column and methanol-water as the mobile phase was established.The α-tocopherol content in millet was determined at 280 nm wavelength with flow rate of 1 mL/min.The results showed that α-tocopherol had a good linearity in the range of 0.5—100 μg/mL with R~2 =0.9993,and the recovery rate was 83%—99%.There were different α-tocopherol contents in the selected 5 varieties of millet,which may be related to the variety.This method is simple,accurate,and can be used for determination of α-tocopherol content in millet.

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