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当前位置: 首页 > 文章 > 高效液相色谱-二极管阵列法测定保健酒中的舒林酸 安徽农业科学 2014 (22) 256-258
Position: Home > Articles > Determination of Sulindac in Health Liquor Using HPLC-PAD Journal of Anhui Agricultural Sciences 2014 (22) 256-258

高效液相色谱-二极管阵列法测定保健酒中的舒林酸

作  者：
郭杰标;蓝献泉;刘旺培;严晓明;何颖娟
单  位：
韶关学院;韶关市食品药品检验所
关键词：
功能食品;舒林酸;违法添加;液-液萃取;色谱检测
摘  要：
[目的]建立保健食品中违禁添加舒林酸的液相检测方法。[方法]通过酸性条件氯仿和碱性水溶液两步液-液萃取,从保健酒中提取和净化舒林酸。使用Kromasil 100-5C18(250×4.6 mm E74572)色谱柱,流动相为0.06 mol/L醋酸缓冲液(pH 4.0)-甲醇-乙腈(59∶29∶12,V/V/V);流速为0.8 ml/min进行分离;检测波长为328 nm。根据色谱峰保留值比对和加标试验,结合二极管阵列扫描图谱对可疑样品定性,并根据外标法峰面积定量检测。[结果]检品和基质成分实现了基线分离,最低检测限为0.5μg/ml,工作曲线的线性范围:10~160μg/ml(R2=0.999 6),回收率88.6%~91.6%。[结论]该研究建立的方法准确、可靠,适用于检测保健品中违法添加的舒林酸。
译  名：
Determination of Sulindac in Health Liquor Using HPLC-PAD
作  者：
GUO Jie-biao;Shaoguan College;
关键词：
Healthy foods;;Sulindac;;Illegal adulterant;;Liquid-liquid extraction;;Chromatographic detection
摘  要：
[Objective]To develop a liquid chromatographic method coupled with photodiode array detector for identification of illegal adulterated sulindac in healthy food. [Method]Two-step liquid-liquid extractions were employed to purify sulindac from samples using chloroform(in acidic condition) following aqueous solution(in alkaline condition),respectively. Chromatographic separation was achieved on a Kromasil 100-5C18column(250 × 4. 6 mm E74572) with the mobile phase of acetonitrile-methanol-0. 06 mol /L acetate buffer(pH 4. 0)(12 /29 /59,V/V/V),at a flow rate of 0. 8 ml /min. The eluant was detected at 328 nm. Retention time and standard addition result combined with PAD-scanning graph were applied to verify sulindac. Quantitation of sulindac was attempted by external standard method. [Result]The results showed as follows: the limit of quantitation was 0. 5 ng /ml. The recovery of sulindac from health liquor was ranging from 88. 6% to 91. 6%. [Conclusion]The method is suitable in determination of sulindac as adulterants in health liquor due to its accuracy and reliability.

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