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Position: Home > Articles > Effect of the Auxin and Ctytokin on the Frequency of Embryogenesis in Kale Acta Horticulturae Sinica 2006,33 (3) 642-644

植物生长调节剂对羽衣甘蓝小孢子胚发生的影响

作  者:
姜凤英;冯辉
单  位:
沈阳农业大学园艺学院
关键词:
羽衣甘蓝;小孢子培养;胚状体;生长素;细胞分裂素
摘  要:
以‘圆叶红心’和‘皱叶红心’2个羽衣甘蓝F1杂交种为试材,进行游离小孢子培养,研究了6-BA、NAA和2,4-D对其胚状体诱导的影响,结果表明:6-BA对诱导胚状体起促进作用,其适宜浓度为0·2mg·L-1;NAA抑制小孢子胚状体发生,但能促进其发育,提高正常胚比例;2,4-D有较强的抑制作用,只有少量细胞团的形成;6-BA、NAA综合作用能显著提高胚状体诱导率,其浓度配比以1∶1或2∶1为佳。
译  名:
Effect of the Auxin and Ctytokin on the Frequency of Embryogenesis in Kale
作  者:
Jiang Fengying~ 1, 2 and Feng Hui~ 1 (~ 1 College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161, China; ~ 2 Floriculture Institute of Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China)
关键词:
Kale; Microspore culture; Embryoid; Auxin; Ctytokin
摘  要:
The effects of 6-BA, NAA and 2, 4-D on embryoid induction during adopting isolated microspore culture of kale (Brassica oleracea L. var. acephala DC.) were studied using two hybrids‘Yuanye Hongxin’and‘Zhouye Hongxin’. The results showed that 6-BA could promote embryoid induction and the suitable content was 0.2 mg·L~ -1 . NAA could not promote embryoid formation, but increased the proportion of normal embryoid. 2, 4-D has significant inhibition to embryoid formation and only produce a little agglomerate cells. In addition, the media with 6-BA and NAA dramatically increased the frequency of microspore-derived embryoids and the suitable content ration was 1∶1 or 2∶1.

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