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当前位置: 首页 > 文章 > HPLC测定红凤菜提取物的总黄酮含量及对提取方法的评价 食品科学 2014,35 (12) 160-164
Position: Home > Articles > Total Flavonoids Determined by HPLC in Extracts of Gynura bicolor DC. FOOD SCIENCE 2014,35 (12) 160-164

HPLC测定红凤菜提取物的总黄酮含量及对提取方法的评价

作  者：
任冰如;吕寒;陈剑;梁呈元;吴菊兰;李维林
单  位：
江苏省中国科学院植物研究所(南京中山植物园),江苏省药用植物研究开发中心,江苏省抗糖尿病药物筛选技术服务中心
关键词：
红凤菜;高效液相色谱法;总黄酮;槲皮素;山柰酚
摘  要：
为了解从红凤菜新鲜茎叶中所得提取物的有效成分含量以及提取方法的分离效果,在提取过程的不同阶段取样得到6个不同提取物,测定其总黄酮含量。首先对提取物进行酸解,再用高效液相色谱法测定槲皮素和山柰酚的含量,根据所测样品中黄酮类成分的组分折算为总黄酮含量。结果显示,经过大孔树脂和聚酰胺2次柱层析后,所得提取物的总黄酮质量分数达到84.8%,重结晶得到的2个结晶物总黄酮质量分数分别为97.1%和99.7%。可见,采用乙醇提取-大孔树脂柱层析-聚酰胺柱层析-重结晶的方法可有效地从红凤菜新鲜茎叶中提取纯化总黄酮。
译  名：
Total Flavonoids Determined by HPLC in Extracts of Gynura bicolor DC.
作  者：
REN Bing-ru;Lü Han;CHEN Jian;LIANG Cheng-yuan;WU Ju-lan;LI Wei-lin;Jiangsu Center for Research & Development of Medicinal Plants, Jiangsu Provincial Service Center for Antidiabetic Drugs Screening,Institute of Botany, Jiangsu Province and Chinese Academy of Sciences/Nanjing Botanical Garden Mem. Sun Yat-Sen.;
关键词：
Gynura bicolor DC.;;high-performance liquid chromatography(HPLC);;total flavonoids;;quercetin;;kaempferol
摘  要：
Six samples collected at different stages during the separation of total flavonoids from crude extracts of the fresh stems and leaves of Gynura bicolor DC. were analyzed for total flavonoid contents. After undergoing acid hydrolysis, the crude extracts were determined for quercetin and kaempferol by high-performance liquid chromatography(HPLC). The amount of total flavonoids was calculated based on the measured flavonoid compounds. After sequential column chromatographies on macroporous resin and polyamide, the total flavonoid content of purified products was 84.8%, and further recrystallization formed two products containing 97.1% and 99.7% total flavoniods, respectively. In conclusion, the process involving ethanol extraction, separation by sequential column chromatographies on macroporous resin and polyamide and recrystallization offers an effective way to purify total flavonoids from the fresh stems and leaves of Gynura bicolor DC.

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