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当前位置: 首页 > 文章 > 灵芝超声波循环提取物成分及活性物质 中国食用菌 2012,31 (3) 47-49
Position: Home > Articles > General Chemical Components and Natural Active Substances in Ultrasonic Circulating Extract Powder of Ganoderma lucidum Edible Fungi of China 2012,31 (3) 47-49

灵芝超声波循环提取物成分及活性物质

作  者：
陈体强;吴岩斌;毛方华;王宏雨
单  位：
福建中医药大学;福建省农业科学院食用菌研究所
关键词：
灵芝;浸膏粉;超声波循环提取;一般化学成分;天然活性成分
摘  要：
采用超声波循环提取获得一种新的灵芝提取物。分析表明,其蛋白质34.41 g.100-1.g-1、可溶性粗多糖40.0 g.100-1.g-1和矿质元素磷1.66 mg.g-1的含量分别高于普通灵芝提取物32.96%、29.45%和30.71%。提取物中均含有18种氨基酸,全氨基酸总量为17.43 g.100-1.g-1,较普通提取物12.95 g.100-1.g-1高34.57%;此外,还检出2种天然活性成分--γ-氨基丁酸和牛磺酸,含量分别为21.92 mg.100-1.g-1和45.97 mg.100-1.g-1。液相质谱分析结果:从提取物中分离检测到至少13个清晰的分子离子峰,m/z范围为266.1~678.5,与香豆素、甾醇类、三萜酸及三萜皂苷等小分子天然活性成分的分子量信息相匹配。
译  名：
General Chemical Components and Natural Active Substances in Ultrasonic Circulating Extract Powder of Ganoderma lucidum
作  者：
CHEN Ti-qiang1 MAO Fang-hua1 WU Yuan-bin2 WANG Hong-yu1(1.Institute of Edible Fungi,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China; 2.Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China)
关键词：
Ganoderma lucidum;extract powder;Ultrasonic circulating extraction;general chemical component;active component
摘  要：
A new extract was prepared by Ultrasonic circulating extraction(UCE),with Ultra-fine particles of log-cultivated fruiting bodies as productive material.Analyzed results showed that content of protein,crude polysaccharide and mineral element P in new extract powder were 34.41 g·100-1·g-1,40.0 g·100-1·g-1and 1.66/mg·g-1,respectively,and higher 32.96%,29.45% and 30.71% than that in ordinary extract powder.There were eighteen kinds of amino acids in the extract powders,and the contents of total amino acids(TAA) and free amino acids(FAA) were 17.43 g·100-1·g-1and 4.28 g·100-1·g-1,with 53.93% ratio of essential amino acids vs total amino acids(E/T=0.5393).Moreover,two active amino acids,γ-Amino butyric acid(GABA) and Taurine(Tau) were checked out,with contents of 21.92 mg·100-1·g-1and 45.97 mg·100-1·g-1 respectively.At least 13 molecular-ions(m/z: 266.1~678.5) were acquired from the extract powder,that were suited with relative molecular molecules of known active compounds,such as coumarin,sterol,triterpene and triterpenoid saponins,by High-performance liquid chromatography coupled with electrospray ionization mass spectrometry(HPLC-ESI-MS)

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