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有机溶剂对多聚半乳糖醛酸酶催化动力学的影响

作  者:
刘小林;李晓婷;龚国勇;黄磊;黄卓烈
单  位:
华南农业大学生命科学学院;江西宜春学院;宜春学院生命科学学院华南农业大学生命科学学院江西宜春中学
关键词:
多聚半乳糖醛酸酶;有机溶剂;动力学
摘  要:
以水溶性低分子质量壳寡糖作为修饰剂对已纯化的多聚半乳糖醛酸酶进行化学修饰,得到化学修饰酶。再以有机溶剂甲醇、乙醇、丙酮、四氢呋喃为效应物,果胶为反应底物,研究其在缓冲液和不同有机溶剂中多聚半乳糖醛酸酶(PG)及其化学修饰酶(COS-PG)的动力学性质。结果表明:修饰后,在缓冲液中COS-PG的Km值有所下降,Vmax上升。在2%的甲醇、乙醇处理后,PG和COS-PG的Km和Vmax均下降。在2%四氢呋喃处理后,PG和COS-PG的Km和Vmax均上升。在2%丙酮处理后,PG和COS-PG的Km上升,而Vmax下降。
译  名:
Effect of Organic Solvent on Catalytic Kinetics of Polygalacturonase
作  者:
LIU Xiao-lin 1,2,LI Xiao-ting 1,GONG Guo-yong 1,HUANG Lei 3,HUANG Zhuo-lie 2,WU Zhi-quan 2,LI Chun-yi 2(1.College of Life Sciences,Yichun University,Yichun 336000,China;2.College of Life Sciences,South China Agricultural University,Guangzhou 510642,China;3.Yichun Middle School,Yichun 336000,China)
关键词:
polygalacturonase;organic solvents;dynamics
摘  要:
Water-soluble chitosan with low molecular weight was employed to modify polygalacturonase(PG).After modification,the catalytic kinetics of PG and modified polygalacturonase(COS-PG) were investigated in buffer,methanol,ethanol,acetone and tetrahydrofuran solution using pectin as substrate.The results indicated that Kmof COS-PG revealed a slight decline,but Vmaxrevealed an increase.Kmand Vmaxof PG and COS-PG declined both in 2% methanol and 2% ethanol.Km and Vmax of PG and COS-PG increased in 2% tetrahydrofuran solution.Conversely,in 2% acetone solution,Km increased but Vmax declined.

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