当前位置: 首页 > 文章 > 菜用黄麻叶蛋白最佳提取工艺研究 北方园艺 2013 (12) 144-147
Position: Home > Articles > 菜用黄麻叶蛋白最佳提取工艺研究 Northern Horticulture 2013 (12) 144-147

菜用黄麻叶蛋白最佳提取工艺研究

作  者:
林燕如;蔡楷钰
单  位:
韩山师范学院化学系
关键词:
菜用黄麻;叶蛋白;提取工艺
摘  要:
以菜用黄麻叶为试材,采用酸提法和加热法制备提取液,在料液比、浸提剂pH值、打浆时间、浸提时间等单因素试验基础上,进行L9(34)正交实验设计,研究了提取菜用黄麻叶蛋白的最佳工艺条件.结果表明:菜用黄麻叶蛋白最佳提取工艺条件为:浸提剂pH 5,打浆时间3min,料液比1∶8,浸提时间6min;叶蛋白的最佳分离条件为:温度85℃时,沉淀分离出叶绿体蛋白质,调节提取液pH 3和11时分别分离出细胞质蛋白质Ⅰ、Ⅱ.菜用黄麻叶蛋白的提取率为8.07%,得率为37.59%.
单  位:
Department of Chemistry,Hanshan Normal University,Chaozhou,Guangdong 521041
关键词:
Corchorus olitorius L.%leaf protein concentrate(LPC)%extraction technology
摘  要:
Taking the leaves of Corchorus olitorius L.as material,on the basis of single factor tests of solid-liquid ratio,pH values of the extracting solvents,pulping time and soaking time,L9(34) orthogonal experiment was used to optimize the extraction conditions of leaf protein concentrate(LPC) using acid method and heating method.The results showed that the best extraction technology was solid-liquid ratio 1∶8,pH values of the extracting solvents 5,pulping time 3 min,soaking time 6 min.The best optimum precipitating conditions were heating temperature 85℃ for the chloroplastic proteins,the pH 3 for cytoplasmic proteins Ⅰ and the pH 11 for the cytoplasmic proteins Ⅱ.Under these conditions,the extraction rate of LPC reached 8.07% and the yield rate was 37.59%.

相似文章

计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊