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当前位置: 首页 > 文章 > 传染性腔上囊病病毒与禽腺病毒混合感染的分子鉴定 中国兽医科学 2009,39 (3) 223-226
Position: Home > Articles > Molecular identification of a coinfection of infectious bursal disease virus with fowl adenovirus Chinese Veterinary Science 2009,39 (3) 223-226

传染性腔上囊病病毒与禽腺病毒混合感染的分子鉴定

作  者：
何秀苗;官丁明;禤金彩;韦平
单  位：
广西民族大学;广西大学
关键词：
传染性腔上囊病病毒;禽腺病毒;VP2蛋白基因高变区;六邻体蛋白基因;混合感染
摘  要：
应用设计的分别针对传染性腔上囊病病毒(IBDV)VP2蛋白基因高变区(vVP2)和Ⅰ群禽腺病毒(FAV)六邻体(hexon)蛋白基因的特异性PCR引物,对临床疑似IBD病例的2只鸡的腔上囊样品和泄殖腔拭子样品分别进行IBDV和FAV的分子检测。结果显示,通过巢式PCR从2份腔上囊样品中均扩增出了471 bp的IBDV vVP2特异性目的片段;SspⅠ和SacⅠ的酶切分析以及序列测定分析证实,该目的片段属超强毒IBDV(very virulent IBDV,vvIBDV)的特征性序列,与常用疫苗株的亲缘关系较远。从2份泄殖腔拭子样品中均扩增出了900 bp的FAV特异性目的片段;序列测定和遗传进化分析表明,该片段在分子水平上属于血清1型FAV(FAV-1)。结果表明,该病例同时感染了vvIBDV和FAV-1。
译  名：
Molecular identification of a coinfection of infectious bursal disease virus with fowl adenovirus
作  者：
HE Xiu-miao1,GUAN Ding-ming1,XUAN Jin-cai1,WEI Ping2(1.Guangxi University for Nationalities,Nanning 530006,China;2.Guangxi University,Nanning 530005,China)
关键词：
infectious bursal disease virus;fowl adenovirus;hypervariable region of VP2 gene;hexon gene;coinfection
摘  要：
Specific PCR primers for hypervariable region of VP2 gene(vVP2) of infectious bursal disease virus(IBDV) and hexon gene of fowl adenovirus(FAV)were designed respectively to detect the genomes of IBDV and FAV from bursal and cloacal swab samples of two individual birds in a flock in which an outbreak of IBD was clinically suspected.A 471 bp-fragment specific for IBDV vVP2 was amplified by a nested PCR from the two samples,and digestions with endonucleases SspⅠ and SacⅠ and nucleotides sequencing showed that the fragment had the sequence characters of very virulent IBDV(vvIBDV) strains and was far from the conventional vaccine viruses in phylogenetic relationship.A 900 bp-fragment specific for hexon gene of FAV was amplified from the cloacal swabs,and the nucleotide sequencing and homology analysis showed that the 900 bp-fragment was molecularly categorized as FAV serotype 1.The results demonstrated that the chicken flock was co-infected by vvIBDV and FAV-1.

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