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Position: Home > Articles > Study on the Genetic Polymorphism of Mitochondrial DNA ND4 Gene of Southwest Horse in China Chinese Journal of Animal and Veterinary Sciences 2009,40 (10) 1473-1477

中国西南马mtDNA ND4基因遗传多样性研究

作  者:
杨虹;赛娜;芒来
单  位:
内蒙古农业大学动物科学与医学学院
关键词:
西南马;线粒体DNA;ND4基因;单倍型;遗传多样性
摘  要:
本研究旨在分析mtDNA ND4基因在中国西南马的遗传多样性。对中国西南马6个品种(类群)142个个体的线粒体DNA ND4基因部分序列进行PCR-SSCP和序列分析。结果表明,拼接后的西南马mtDNA ND4基因序列大小为679 bp,A+T含量(55.7%)高于G+C含量(44.3%)。共检测到11个核苷酸变异位点,这些变异位点可归纳为6种单倍型。核苷酸平均多样度为0.638%,表明受试西南马mt DNA遗传多样性并不十分丰富。对各单倍型进行聚类分析,结果提示中国西南马起源于2个母系祖先。
译  名:
Study on the Genetic Polymorphism of Mitochondrial DNA ND4 Gene of Southwest Horse in China
作  者:
YANG Hong,SAI Na,MANG Lai(College of Animal Science and Animal Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China)
关键词:
Southwest horse;mitochondrial DNA;ND4 gene;haplotype;genetic polymorphism
摘  要:
This experiment is aimed to analyze mitochondrial DNA ND4 gene genetic polymorphism of Southwest horse in China.A partial fragment of mitochondrial DNA ND4 gene was sequenced and analyzed by PCR-SSCP for 142 individuals from 6 Southwest horse breeds in China.The results showed that the spliced sequence of mtDNA ND4 gene of Southwest horse was 679 bp,and the content of A+T(55.7%) was higher than that of G+C(44.3%).11 mutational sites were detected and all of these sequence polymorphic sites were sorted into 6 haplotypes.The average nucleotide diversity was 0.638%,and the genetic polymorphism of mtDNA of tested Southwest horse was not abundant.The cluster analysis of these haplotypes suggested that Southwest horse in China originated from two maternal ancestors.

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