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Position: Home > Articles > Phylogenetic Relationship of Pleurotus Species Based on Nuclear Large Subunit Ribosomal DNA Sequences Journal of Plant Genetic Resources 2008,9 (3) 328-334

基于nLSU rDNA序列分析探讨侧耳属系统发育关系

作  者:
高山;黄晨阳;陈强;边银丙;张金霞
单  位:
中国农业科学院农业资源与农业区划研究所;华中农业大学应用真菌研究所
关键词:
侧耳属;核糖体大亚基;分子系统发育;多系起源;菌丝系统
摘  要:
以PCR产物直接测序分析了侧耳属(Pleurotus)27个种41个菌株的nLSU rDNA序列,以Hohenbuehelia serotina和Lentinula edodes为外群,运用MEGA3.1软件中的邻接法(neighbor-joining)和最大简约法(maximums parsimony)分别构建侧耳属的系统发育树。分子系统发育树表明:Coremiopleurotus组和Pleurotus属内单、二系菌丝系统均为多系起源;P. rattenburyi,刺芹侧耳种族群(P. eryngiispecies-complex)与被划分在Lepiotarii组的栎侧耳P. dryinus能与侧耳属其他成员明显分开;红侧耳P. djamor、桃红侧耳P. salmoneostramineus、扇形侧耳P. flabellatus与贝盖侧耳P. calyptratus关系密切;金顶侧耳P. citrino-pileatus与P. euosmus聚在一起,而与黄白侧耳P. conucopiae为不同的分类单元;肺形侧耳P. pulmonarius与P. abieticale亲缘关系较近。
译  名:
Phylogenetic Relationship of Pleurotus Species Based on Nuclear Large Subunit Ribosomal DNA Sequences
作  者:
GAO Shan1,2,3,HUANG Chen-yang2,CHEN Qiang2,BIAN Yin-bing1,ZHANG Jin-xia2(1Institute of Applied Mycology,Huazhong Agricultural University,Wuhan 430070;2Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081;3Hubei Institute for Nationalities,Enshi 445000)
关键词:
Pleurotus;nLSU rDNA;Molecular phylogeny;Polyphyletic;Hyphal systems
摘  要:
Phylogenetic relationship of the genus Pleurotus was conducted by direct sequencing nLSU rDNA from 41 strains in 27 Pleurotus species.The phylogenetic tree of Pleurotus was assembled by software MEGA version 3.1 with neighbor-joining and maximums parsimony with outgroup Hohenbuehelia serotina and Lentinula edodes.The phylogenetic tree showed that monomitic and dimitic hyphal systems were polyphyletic for the Coremiopleurotus group.Molecular phylogeny also suggested that P.rattenburyi,P.eryngii species-complex and P.dryinus of Lepiotarii group could be distinguished from other species of Pleurotus,respectively.P.djamor,P.salmoneostramineus,P.flabellatus and P.calyptratus,P.pulmonarius and P.abieticale,P.citrinopileatus and P.euosmus had close relation.P.citrinopileatus and P.conucopiae were not the same Pleurotus species.

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