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Position: Home > Articles > Several Key Problems on Two-dimensional Electrophoresis Biotechnology Bulletin 2005,0 (6) 62-64

双向电泳应注意的几个关键问题

作  者:
王凤茹
单  位:
中国科学院植物研究所发育中心
关键词:
蛋白组;双向电泳;固相pH梯度等电聚焦;SDS-PAGE
摘  要:
着重介绍双向电泳的关键技术,如样品的提取,电泳条件的选择及双向电泳中应注意的关键问题等,为蛋白组学工作者提供可以借鉴的方法和经验。
译  名:
Several Key Problems on Two-dimensional Electrophoresis
作  者:
Wang Fengru~(1,2)(~(1)Institute of Botany,The Chinese Academy of Scienses,Beijing 100093;~(2)Agricultural University of HeBei,Baoding 071001)
关键词:
Proteome Two-dimensional electrophoresis IPG SDS-PAGE
摘  要:
Combining the author's working experiences,we introduce here several key techniques on the two-dimensional electrophoresis,such as the extraction of sample; the choices of the condition of two-dimensional electrophoresis and what should be pay attention to.We hope to provide some methodologies for those who do research in proteomes.

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