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Position: Home > Articles > Isolation and identification of probiotics from swine intestine and its biological characteristics Animal Husbandry & Veterinary Medicine 2012,44 (1) 19-23

1株猪源肠道益生菌的分离鉴定及其生物学特性研究

作  者:
高书锋;刘惠知;周映华;缪东;吴胜莲;周小玲
单  位:
湖南省微生物研究所
关键词:
凝结芽孢杆菌;鉴定;猪肠道;生物学特性
摘  要:
从健康猪肠道中分离出1株对仔猪大肠杆菌病和副伤寒病病原有较强拮抗作用的菌株,命名为W-02,对其进行菌落形态、生理生化特性和16S rDNA系统发育分析,确定该菌株为凝结芽孢杆菌。对该菌株胃肠道耐受特性和安全性进行了研究,结果表明,W-02菌株在人工胃液、人工肠液、猪胆盐和80℃高温表现出很强的耐受能力,急性毒性试验结果表明该菌株为无毒菌株。
译  名:
Isolation and identification of probiotics from swine intestine and its biological characteristics
作  者:
GAO Shu-feng,LIU Hui-zhi,ZHOU Ying-hua, MIAO Dong,WU Sheng-lian,ZHOU Xiao-ling(Hunan Institute of Microbiology,Changsha 410009,China)
关键词:
Bacillus coagulan;identification;swine intestine;biological characteristics
摘  要:
A bacterial strain,named W-02,with strong antagonistic effect on pathogens of swine colibacilosis and salmonellosis was isolated from healthy swine intestine.On the basis of bacterial culture,physiological and biochemical characteristics and 16S rDNA sequences analysis,W-02 strain was identified as Bacillus coagulan.After being inoculated in artificial gastric juice and intestinal liquid for different time,W-02 strain showed stronger tolerance.And the bacterial strain had higher tolerance to different swine bile concentrations and high temperature of 80℃ for different time.Acute toxicity test demonstrated W-02 strain to be avirulent.

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