关键词：
台湾金线莲;组培苗规格;栽培基质;成活率;鲜重增殖倍数;折干率
摘 要：
【目的】筛选适合台湾金线莲组培苗生长的移栽规格及栽培基质,为优化金线莲栽培技术提供参考依据。【方法】选取很小苗、小苗、中等苗、大苗和超大苗5种规格的台湾金线莲组培苗、7种不同配方的栽培基质,进行台湾金线莲组培苗生长状况比较试验,测定其定植1和6个月的成活率、6个月的鲜重增殖倍数及折干率。【结果】5种规格台湾金线莲组培苗定植后1和6个月,成活率均高于90.0%。小规格台湾金线莲组培苗定植后其鲜重增殖倍数明显高于大规格苗,5种规格组培苗的鲜重增殖倍数排序为:很小苗>小苗>大苗>中等苗>超大苗;中小规格苗的折干率比大苗高,其排序为:中等苗>小苗>很小苗>大苗>超大苗。以水草为栽培基质,植株鲜重增殖倍数最高,为1.96倍,折干率最小,为8.8%,但组培苗定植操作速度慢;以Klasmann泥炭土422#为栽培基质,植株成活率和折干率均最高,分别为100.0%和10.6%,但栽培成本较高;林下腐殖土基质的栽培成本较低,栽培效果居中上水平。【结论】小规格台湾金线莲组培苗栽培效果优于大苗;以Klasmann泥炭土422#和林下腐殖土为基质栽培效果较好;林下腐殖土成本低,可作为山区农村栽培金线莲的首选基质。
译 名：
Screening for tissue-cultured plantlet transplanting size and cultivation medium of Anoectochilus formosanus
作 者：
WEI Cui-hua;XIE Yu;QIN Jian-bin;CHEN Qin;Fuzhou Institute of Agricultural Sciences;
关键词：
A.formosanus;;size of tissue-cultured plantlet;;cultivation medium;;survival rate;;proliferation rate of fresh weight;;DW/FW
摘 要：
【Objective】The tissue-cultured plantlets transplanting size and cultivation medium of Anoectochilus formosanus were screened, in order to provide references for optimizing cultivation techniques of A. formosanus. 【Method】Taking A. formosanus plantlets with 5 sizes(smaller-sized plantlets, small-sized plantlets, medium-sized plantlets, bigsized plantlets and bigger-sized plantlets) as materials, the field-planting experiment was carried out to compare growth status of A. formosanus by using 7 kinds of cultivation media with different formulas. Meanwhile, the survival rate of plantlets in vitro after field-planting for 1 month and 6 months, the proliferation rate of fresh weight and the dry weight/fresh weight ratio(DW/FW) after field-planting for 6 months were determined. 【Result 】The results showed that, the survival rate of A. formosanus plantlets with 5 sizes after field-planting reached up to 90.0%, the proliferation rate of fresh weight for small-sized plantlets were significantly higher than the big-sized ones, and A. formosanus plantlets with different sizes were ranked according to their proliferation rates of fresh weight, as follows: smaller-sized plantlets >small-sized plantlets > big-sized plantlets > medium-sized plantlets > bigger-sized plantlets. Moreover, the DW/FW of small and medium-sized plantlets were higher than big-sized ones, and A. formosanus plantlets with different sizes were ranked according to DW/FW, as follows: medium-sized plantlets > small-sized plantlets > smaller-sized plantlets > big-sized plantlets > bigger-sized plantlets. In addition, the plantlets planted in cultivation medium composed of aquatic weed had the maximal proliferation rate(1.96) of fresh weight and the minimal DW/FW(8.8%), but the planting speed of plantlets in vitro was slow. The plantlets planted in cultivation medium composed of Klasmann peat 422# had the maximal survival rate(100.0%) and DW/FW(10.6%), but the its cultivation cost was higher. However, the cultivation cost of plantlets planted in cultivation medium composed of forest humus soil was lower, and the cultivation effect were above average.【Conclusion】The cultivation effect of small-sized A. formosanus plantlets in vitro was better than that of big-sized ones.And the cultivation media composed of Klasmann peat 422# and forest humus soil show better cultivation effect. Furthermore, the low-cost forest humus soil can be used as preferred cultivation medium of A. formosanus in the mountainous rural area.
