当前位置: 首页 > 文章 > 赤点石斑鱼4种同工酶的组织分布及基因位点分析 浙江海洋学院学报(自然科学版) 2004,23 (2) 103-106
Position: Home > Articles > Analysis on Tissue Distributions of Four Kinds of Isozyme and Their Loci of Epinephelus akaara Journal of Zhejiang Ocean University(Natural Science) 2004,23 (2) 103-106

赤点石斑鱼4种同工酶的组织分布及基因位点分析

作  者:
邓思平;刘楚吾
单  位:
湛江海洋大学水产学院
关键词:
赤点石斑鱼;同工酶;电泳;组织
摘  要:
运用聚丙烯酰胺垂直梯度凝胶电泳法对赤点石斑鱼7组织(肌肉、肝脏、肾脏、心肌、脑、脾脏、性腺)的4种同工酶(EST、LDH、MDH、ME)进行了初步研究,并对4种酶的同工酶位点及酶谱表型进行了分析。结果表明:赤点石斑鱼的4种同工酶系统具有明显的组织特异性;EST由4个基因位点编码;LDH酶带多于典型的5条酶带;MDH具有线粒体型(m-MDH)和上清液型(s-MDH)两种类型;7种组织只检测到一种类型的ME。
译  名:
Analysis on Tissue Distributions of Four Kinds of Isozyme and Their Loci of Epinephelus akaara
作  者:
DENG Si-ping,LIU Chu-wu(Fisheries College of Zhanjiang Ocean University,Zhanjiang 524025,China)
关键词:
Epinephelus akaara;isozyme;electrophoresis;tissue
摘  要:
By the vertical polyacrylamide gel gradient electrophoresis four isozyme (EST、LDH、MDH、ME) from seven tissues(muscle,liver,kidney,heart,brain,s pleen,gonad) of Epinephelus akaara were studied, and the loci and phenotypes wer e also analyzed.The results showed that all isozyme presented tissue specificity . EST encoded by four loci.LDH enzymatic band was more than 5 of typical teleost LDH phenotypes.Two kinds of MDH(m-MDH,s-MDH) and one type of ME were found in E.akaara.

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