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当前位置: 首页 > 文章 > 单环刺螠体壁多糖的分离纯化、理化性质及抗脂质过氧化活性 食品科学 2015,36 (8) 162-166
Position: Home > Articles > Purification, Physicochemical Properties and Anti-Lipid Peroxidation Activity of Polysaccharides from Body of Urechis unicinctus FOOD SCIENCE 2015,36 (8) 162-166

单环刺螠体壁多糖的分离纯化、理化性质及抗脂质过氧化活性

作  者：
朱佳利;陈依莎;牛庆凤;李涛;陈荫
单  位：
浙江海洋学院食品与医药学院
关键词：
单环刺螠;多糖;分离纯化;理化性质;抗氧化
摘  要：
为研究和开发单环刺螠中活性多糖成分,采用酶法提取和色谱分离技术,从单环刺螠的体壁中提取分离多糖组分,采用化学和仪器分析方法对其单糖组成、分子质量等理化性质进行研究,并通过体外清除脂质过氧化物实验对其进行初步的活性评价。结果表明:采用木瓜蛋白酶和胰蛋白酶两步酶解后,单环刺螠多糖(unicinctus polysaccharide,UP)得率为5.3%。通过离子交换柱层析分离纯化后主要得到两个组分UP-1和UP-2。UP-1是主要由葡萄糖(Glc)组成的高聚葡聚糖,分子质量340 k D,而UP-2为组成复杂的类糖胺聚糖,分子质量约为7.9 k D,主要含有葡萄糖(Glc)、半乳糖(Gal)、氨基葡萄糖(Glc N)和岩藻糖(Fuc),还含有少量的甘露糖(Man)和氨基半乳糖(Gal N)。其单糖组成Man、Glc N、Gal N、Glc、Gal、Fuc物质的量比例为1.0∶1.47∶0.64∶6.49∶2.5∶3.0。和其他海洋动物来源类糖胺聚糖类似,UP-2还含有少量的硫酸基,含量为7.4%。对UP-1和UP-2进行初步体外抗氧化活性研究表明,类糖胺聚糖UP-2具有显著的脂质过氧化物清除活性,半数清除质量浓度为5.0 mg/m L。
译  名：
Purification, Physicochemical Properties and Anti-Lipid Peroxidation Activity of Polysaccharides from Body of Urechis unicinctus
作  者：
ZHU Jiali;CHEN Yisha;NIU Qingfeng;LI Tao;CHEN Yin;School of Food and Pharmacy, Zhejiang Ocean University;
关键词：
Urechis unicinctus;;polysaccharide;;purification;;physicochemical property;;antioxidant activity
摘  要：
This study aimed to extract polysaccharides from the body wall of Urechis unicinctus by sequential enzymatic digestion with papain and trypsin and to purify the crude extract by anion exchange column chromatography. The physical and chemical properties such as monosaccharide composition and molecular weight of the purified polysaccharides were investigated on the basis of chemical and modern spectroscopic analysis. Then the antioxidant activity in vitro of the polysaccharides was evaluated. The results showed that the yield of polysaccharides extracted from the body wall of Urechis unicinctus was 5.3%. Two polysaccharides, namely UP-1 and UP-2, were purified from the crude polysaccharides by anionexchange chromatography. UP-1 was a neutral polysaccharide composed of mainly glucose with a molecular weight of 340 k D. UP-2 was a heteropolysaccharide with a molecular weight of about 7.9 k D. UP-2 consisted of mannose, glucosamine, galactosamine, glucose, galactose and fucose at a molar ratio of 1.0:1.47:0.64:6.49:2.5:3.0. The monosaccharide composition revealed that UP-2 was a glycosaminoglycan-like polysaccharide. Like most of glycosaminoglycan-like polysaccharides from marine animals, UP-2 contained 7.4% sulfate ester. The glycosaminoglycan-like polysaccharide had a wide range of bioactivities and better anti-lipid peroxidation activity than UP-1 as evidenced by its EC50 value at 5.0 mg/m L.

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