作 者：
李磊;沈新强;李超;王云龙;蒋玫;许高鹏
单 位：
中国水产科学研究院东海水产研究所;上海海洋大学海洋科学学院
摘 要：
为了研究苯并[a]芘(Bap)、菲PHE在缢蛏体内的富集及释放过程的动力学特征,通过双箱动力学模型对富集(15 d)与释放(9 d)过程进行非线性曲线拟合,获得缢蛏对Ba P、PHE的吸收速率常数k1、释放速率常数k2、生物富集因子BCF、平衡状态下生物体内Ba P含量CAmax、生物学半衰期B1/2。结果显示,缢蛏对Ba P的富集能力高于PHE,Ba P增加幅度分别为0.45 ng/(kg·d)(40μg/L实验组)、0.33 ng/(kg·d)(20μg/L实验组)、0.23 ng/(kg·d)(10μg/L实验组);PHE增加幅度分别为0.33 ng/(kg·d)(30μg/L实验组)、0.25 ng/(kg·d)(20μg/L实验组)、0.18 ng/(kg·d)(10μg/L实验组)。Ba P、PHE的富集、清水释放均为前期迅速,后期缓慢。缢蛏对Ba P、PHE的k1范围分别为0.39~0.52,0.30~0.43,平均值分别为0.44和0.36,均随Ba P、PHE暴露浓度的增大而减少;k2范围分别为0.001 6~0.001 7,0.002 8~0.003 3,平均值分别为0.001 6和0.003 0,无明显的变化趋势;BCF范围分别为243.96~306.28,105.73~130.85,平均值分别为268.18和118.57,均随Ba P、PHE暴露浓度的增大而减少;CAmax范围分别为3 062.76~9 758.25μg/kg,1 308.54~3 171.84μg/kg,平均值分别为5 969.08和2 287.71μg/kg;B1/2范围分别为407.73~433.22 d,210.68~248.06 d,平均值分别为421.20和232.04 d,均随Ba P、PHE暴露浓度的增大而增大。
译 名：
Bioaccumulation-depuration kinetics of benzo [a] pyrene and phenanthrene in Sinonovacula constricta
作 者：
LI Lei;SHEN Xinqiang;LI Chao;WANG Yunlong;JIANG Mei;XU Gaopeng;East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;College of Marine Sciences,Shanghai Ocean University;
关键词：
Sinonovacula constricta;;benzo[a]pyrene;;phenanthrene;;bioaccumulation
摘 要：
In this study,marine bivalve molluscs species,Sinonovacula constricta,were exposed to benzo[a]pyrene( Ba P) and phenanthrene( PHE) due to their ubiquitousness and bioavailability in the marine aquatic environment. Kinetic parameters( uptake rate constant( k1),elimination rate constant( k2),accumulation factor( BCF),and biological half-life( B1 /2)) of the Ba P and PHE bioaccumulation and depuration were calculated by two-compartment kinetic model for 15-day uptake and subsequent 9-day depuration periods.The results show ed that the bioaccumulation ability of Ba P was higher than PHE in S. constricta during 15-day uptake period. The increase of Ba P was 0. 45 ng /( kg ·d)( 40 μg / L groups),0. 33 ng /( kg ·d)( 20μg / L groups),0. 23 ng /( kg·d)( 10 μg /L groups),respectively. The increase of PHE was 0. 33 ng /( kg·d)( 30 μg / L groups),0. 25 ng /( kg · d)( 20 μg / L groups),0. 18 ng( kg · d)( 10 μg / L groups),respectively. The uptake and depuration periods of Ba P and PHE were mainly in the initial stage of experiment. Those values of k1 of Ba P and PHE were calculated as varying from 0. 39 to 0. 52 and from 0. 30 to 0. 43,meanw hile,the average values of k1 were 0. 44 and 0. 36,respectively. Those values of k1 decreased with the increase of Ba P and PHE exposure concentration in ambient seaw ater. Those values of k2 of Ba P and PHE were calculated as varying from 0. 001 6 to 0. 001 7 and from 0. 002 8 to 0. 003 3,meanw hile,the average values of k2 were 0. 001 6 and 0. 003 0,respectively. There was no obvious variation trend of those values of k2. Those values of CAmaxof Ba P and PHE were calculated as varying from 3 062. 76 to 9 758. 25μg /kg and from 1 308. 54 to 3 171. 84 μg /kg,meanwhile,the average values of CAmaxwere 5 969. 08 and2 287. 71 μg / kg,respectively. Those values of B1 /2of Ba P and PHE were calculated as varying from 407. 73 to 433. 22 d and from 210. 68 to 248. 06 d,meanw hile,the average values of B1 /2w ere 421. 20 d and232. 04 d,respectively. Those values of CAmaxand B1 /2increased with the increase of Ba P and PHE exposure concentration in ambient seawater.
