关键词:
低频磁场;紫色红曲菌;生物量;麦角固醇;液态发酵
摘 要:
探讨低频磁场处理对紫色红曲菌(Monascus purpurcus)发酵产麦角固醇的影响,确定最佳处理条件。分别研究低频磁场强度、处理时间和处理时期对紫色红曲霉液态发酵产麦角固醇的影响规律,探讨在最佳处理条件下,磁场对紫色红曲霉麦角固醇代谢过程的影响和紫色红曲霉的生物量在整个发酵周期的变化,及对紫红曲霉其他主要代谢物的影响。结果表明:低频磁场处理紫色红曲菌的最适条件为磁场强度1.2 mT、处理时间2 d、处理时期在发酵0~2 d,在发酵第12天,麦角固醇产量达到了7.08 mg/g,生物量达到3.2 g/L,比对照组分别增加了62.5%和31.68%。低频磁场是提高紫色红曲菌生产麦角固醇产量的有效手段,研究为低频磁场辅助微生物发酵,提高有益产物的合成提供了数据支持。
译 名:
Effect of Low-Frequency Magnetic Field on Ergosterol Production by Monascus purpureus in Liquid-State Fermentation
作 者:
WAN Yunlei;HAN Hongxia;LI Li;WU Xiaojie;GAO Mengxiang;College of Life Science,Yangtze University;Jingchu Food Research and Development Center,Yangtze University;
关键词:
low-frequency magnetic field(LF-MF);;Monascus purpurcus;;biomass;;ergosterol;;liquid-state fermentation
摘 要:
We explored the effects of low-frequency magnetic field(LF-MF) on the production of ergosterol by fermentation with Monascus purpurcus and further determined the optimal LF-MF treatment conditions such as LF-MF intensity, time treatment time and the fermentation stage when M. purpurcus was treated with LF-MF on ergosterol production by liquid-state fermentation of M. purpurcus. We also investigated the effects of LF-MF on the metabolism of ergosterol by M. purpurcus, and examined the change in M. purpurcus biomass throughout the fermentation period and the effects on other main metabolites of M. purpurcus under optimal treatment conditions. Results showed that ergosterol yield was 7.08 mg/g and the biomass of M. purpurcus was 3.2 g/L on the 12~(th) day of fermentation, which were respectively increased by 62.5% and 31.68% as compared to that of the control when M. purpurcus was treated by LF-MF at an intensity of 1.2 m T during the first two days of fermentation. Consequently, LF-MF was an effective method to enhance ergosterol yield. This result may provide evidence supporting the use of LF-MF to improve the production of beneficial microbial metabolites by fermentation.