单 位：
甘肃省干旱生境作物学重点实验室;甘肃农业大学工学院;甘肃农业大学;甘肃农业大学生命科学技术学院
关键词：
雾培马铃薯;幼苗;黄腐酸;聚乙二醇6000;干旱胁迫;生长生理;抗性
摘 要：
试验以马铃薯(Solanum tuberosum)幼苗为研究对象,采用雾培装置进行不同黄腐酸用量和干旱胁迫处理(分别为CK,不加黄腐酸和PEG;10%PEG;0.01%FA+10%PEG;0.03%FA+10%PEG;0.05%FA+10%PEG和0.10%FA+10%PEG),分析其对马铃薯生长发育及抗性生理的影响。结果表明,在干旱胁迫下,雾培马铃薯幼苗的成活率、叶绿素含量、膜稳定指数、根系活力、匍匐茎数、结薯率和产量呈下降趋势,而丙二醛(MDA)、过氧化氢(H2O2)、脯氨酸、可溶性糖含量和过氧化氢酶(CAT)活性及超氧阴离子(O.2)产生速率呈上升趋势,过氧化物歧化酶(SOD)和过氧化物酶(POD)活性先增加后下降,说明雾培马铃薯幼苗在干旱胁迫下生长发育受到显著抑制,正在遭受逆境胁迫。经黄腐酸处理后,幼苗的成活率、匍匐茎数、结薯率和产量则得到了提高,而叶绿素含量、膜稳定指数和根系活力仍呈下降趋势,但较单一干旱胁迫的下降趋势缓慢,而MDA含量、SOD和POD活性及产生速率呈先升高后下降的趋势,脯氨酸、可溶性糖、H2O2含量及CAT活性呈上升趋势,其中H2O2含量上升趋势较单一干旱胁迫缓慢,说明黄腐酸处理后使干旱胁迫下雾培马铃薯幼苗的生长发育得到了促进,减轻了干旱胁迫对幼苗造成的伤害,提高了植株的整体抗旱性,而这种抗性与黄腐酸浓度呈明显相关性,0.05%FA对植株的的保护效应最显著,而0.10%FA则协同PEG加剧了对幼苗的伤害作用。
译 名：
Influences of fulvic acid on drought resistance of aeroponic potato seedlings
作 者：
QIU Meng-ke1,HUI Zhen-long2,3,HUANG Xiao-peng1,WU Jin-feng1(1.College of Engineering,Gansu Agricultural University,Lanzhou,Gansu 730070,China;2.College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;3.Gansu Provincial Key Laboratory of Aridland Crop Science,Gansu Agricultural University,Lanzhou,Gansu 730070,China)
关键词：
aeroponic potato;seedling;fulvic acid;PEG-6000;drought stress;growth and physiological parameter;resistance
摘 要：
Using potato(Solanum tuberosum) seedlings as research objects,effects of different treatments of fulvic acid and drought stress(CK,10% PEG,0.01% FA+10 % PEG,0.03% FA+10% PEG,0.05% FA+10% PEG,and 0.10% FA+10% PEG) on growth and drought-resistant physiological parameters of aeroponic potato were studied.The results showed that seedling survival rate,chlorophyll content,membrane stability index,root vigor,number of procumbent stems,tuber rate and output of aeroponic potato tended to decrease,malondialdehyde(MDA) content,hydrogen peroxide(H2O2) content,proline content,soluble sugar content,catalase(CAT) activity and production rate of superoxide anion(O ·2) tended to increase,and activity of superoxide dismutase(SOD) and peroxidase(POD) increased firstly and then decreased under drought stress.Thus the growth and development of aeroponic potato seedlings were inhibited significantly under drought stress.Under the treatments of fulvic acid and drought stress,seedling survival rate,number of procumbent stems,tuber rate and output increased,while chlorophyll content,membrane stability index and root vigor still tended to decrease but their decrease rate was smaller than that under treatment of single drought stress;MDA content,SOD activity and POD activity increased firstly and then decreased;Proline content,soluble sugar content,H2O2 content and catalase(CAT) activity increased,in which the increase rate of H2O2 content was lower than that under the treatment of single drought stress.Therefore,application of fulvic acid could promote growth and development of aeroponic potato seedlings under drought stress,and improve overall drought resistance of plants.The resistance was significantly associated with concentration of fulvic acid.The protective effect of 0.05% FA was the most significant,but 0.10% FA even brought cooperatively injury to potato seedlings with PEG stress.
