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Position: Home > Articles > Association between Genetic Diversity of MHC Bloodtype and Some Resistant Traits of Chicken China Poultry 2012,34 (24) 21-24

鸡MHC多态性及血型与部分免疫指标的关联分析

作  者:
翟飞;马腾;陈东升;周伟;程立力;张康宁;袁青妍;许盛海;嵇宝华;常国斌;陈国宏
单  位:
常州市立华畜禽有限公司;连云港东米食品有限公司;扬州大学动物科学与科技学院
关键词:
微卫星;MHC;血型;抗性
摘  要:
利用荧光标记微卫星技术对雪山鸡MHC基因Ⅰ~Ⅳ区进行遗传多样性及血型判断进行检测,并分析不同血型与部分抗性性状的相关性。结果发现:雪山鸡MHC-Ⅰ基因观察杂合度为0.6075,多态信息含量为0.5382;MHC-Ⅳ基因观察杂合度为0.7975,多态信息含量为0.7668。雪山鸡血型较为复杂,主要血型有B2、B5、B13、B21、B22、B76。其中,B2综合抗性指标显著好于其他血型(P<0.05)。该结果为利用血型进行抗病育种提供了基础资料。
译  名:
Association between Genetic Diversity of MHC Bloodtype and Some Resistant Traits of Chicken
作  者:
ZHAI Fei1,MA Teng1,CHEN Dongsheng2,ZHOU Wei1,CHENG Lili3,ZHANG Kangning3,YUAN QingYan3,XU Shenghai3,JI Baohua3,CHANG Guobin1,CHEN Guohong1(1.College of Animal Science and Technology,Yangzhou University,Yangzhou,Jiangsu 225009;2.Lianyungang Dongmi Food Co.,Ltd.,Lianyungang,Jiangsu 222248;3.Lihua Livestock Co.,Ltd.,Changzhou,Jiangsu 213100)
关键词:
microsatellite;MHC;bloodtype;resistant traits
摘  要:
This paper was designed to study the genetic diversity of MHC and bloodtype identification and its association with some resistant traits of Xueshan chicken.With microsatellite DNA markers,the genetic diversity of MHCⅠ to Ⅳ,and according to microsatellite locus LEI0258 and MCW0371 bloodtype was detected.The results showed that average observed heterozygosity on locus of MHC-Ⅳ was 0.7975,the average polymorphic information content was 0.7668,while on locus of MHC-Ⅰ was 0.6075 and 0.5383.In Xueshan chicken,six genotypes(B2,B13,B21,B5,B22,B76)were found,and the association analysis with resistant traits found that B21 was the best bloodtype.This paper provided the reference data for disease-resistant breeding association with bloodtype.

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