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Monitoring Soil Microbial Activities in Different Cropping Systems Using Combined Methods

作  者:
Zhimin Yuan;Haijun Liu;Jun Han;Jian Sun;Xiaoshuai Wu;Jun Ya
单  位:
School of Energy & Environmental Enginering, National International Cooperation Base on Environment and Engery, University of Science and Technology Beijing, Beijing 100083 (China);School of Resources and Environment, Anqing Normal University, Anqing 246133 (China); School of Water Resource and Environmental Engineering, Sino–Hungarian Joint Laboratory of Environmental Science and Health, China University of Geosciences, Beijing 100083 (China
关键词:
activities;soil;05;r =;cropping;positive correlatio
摘  要:
Cropping activities may affect soil microbial activities and biomass, which would affect C and N cycling in soil and thus the crop yields and quality. In the present study, a combination of microcalorimetric, enzyme activity (sucrase, urease, catalase, and fluorescein diacetate hydrolysis), and real-time polyrnerase chain reaction (RT-PCR) analyses was used to investigate microbial status of farmland soils, collected from 5 different sites in Huazhong Agriculture University, China. Our results showed that among the 5 sites, both positive and negative impacts of cropping activities on soil microbial activity were observed. Enzyme activity analysis showed that cropping activities reduced soil sucrase and urease activities, which would influence the C and N cycles in soil. Much more attentions should be given to microbial status affected by cropping activities in future. According to the correlation analysis, fluorescein diacetate hydrolysis showed a significantly (P < 0.05) negative correlation with the time to reach the maximum power output (R = 0.898), but a significantly (P < 0.05) positive correlation with bacterial gene copy number (R = 0.817). Soil catalase activity also showed a significantly (P < 0.05) positive correlation with bacterial gene copy number (R = 0.965). Using combined methods would provide virtual information of soil microbial status.

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