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Research Progress on Agrobacterium tumefaciens-based Transgenic Technology in Brassica rapa

作者：

Guoliang Li;Lixin Yue;Fēi Li;Shifan Zhang;Hui Zhang;Wei Qian;Zhiyuan Fang;Jian Wu;Xiaowu Wang;Shujiang Zhang;Rifei Su

关键词：

Brassica rapa;Agrobacterium tumefaciens;transgenic;transformation;IMMEDIATE COLCHICINE TREATMENT;INFLUENCING SHOOT REGENERATION;CHINESE-CABBAGE PLANTS;DOUBLED HAPLOID PLANTS;MEDIATED TRANSFORMATION;COTYLEDONARY EXPLANTS;GENETIC-TRANSFORMATION;SOMATIC EMBRYOGENESIS;ISOLATED MICROSPORES;PROTOPLAST CULTURE

摘要：

Brassica rapa L. is cultivated globally and consumed in many areas worldwide. Using the transgenic Agrobacterium-mediated transformation method, which is a reproducible and efficient technique, genes can be transferred into various B. rapa species. This review summarizes the processes involved in Agrobacterium-mediated transformation of B. rapa, including surface seed sterilization, co-cultivation with A. tumefaciens, induction of callus/shoot/root formation, and confirmation of transgenic plants. In addition, factors such as the Agrobacterium strain, plant genotype, explant age, transformation efficiency of the hybrid or inbred line, and the concentrations of N6-benzyl amino purine and naphthalene acetic acid, are discussed. And this review shows clearly how to do it, what to do, and what not to do in the transgenic Agrobacterium-mediated in Brassica rapa. The information presented here lays the foundation for a simple and efficient method that resolves existing problems and improves overall transgenic B. rapa production, thereby benefiting both basic and applied research.

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Research Progress on Agrobacterium tumefaciens-based Transgenic Technology in Brassica rapa

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