中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Selenium reduces hepatopancreas lipid accumulation of grass carp (Ctenopharyngodon idella) fed high-fat diet via lipophagy activation. 动物营养(英文) 2023,15

Position: Home > Articles > Selenium reduces hepatopancreas lipid accumulation of grass carp (Ctenopharyngodon idella) fed high-fat diet via lipophagy activation. Animal Nutrition 2023,15

Selenium reduces hepatopancreas lipid accumulation of grass carp (Ctenopharyngodon idella) fed high-fat diet via lipophagy activation.

作者：

Xiaotian Zhang;Haibo Yu;Xianfang Yan;Pengju Li;Chi Wang;Cheng Zhang;Hong J

关键词：

Grass carp;High-fat diet;Lipid metabolism;Lipophagy;Seleniu

摘要：

It has been reported that selenium (Se) can reduce hepatopancreas lipid accumulation induced by high-fat diet. However, its mechanism is still unknown. This study aims to investigate the specific mechanisms by which Se alleviates high-fat diet-induced lipid accumulation. Grass carp were fed control diet (4.8% lipid, Con), high-fat diet (8.8% lipid, HFD) or HFD supplemented with 0.3 mg/kg nano-Se (HSe0.3) for 10 weeks. Growth performance, Se deposition, lipid accumulation, hepatic ultrastructure, and gene and protein expression levels associated with autophagy were examined. Furthermore, oleic acid (OA) was used to incubate the grass carp hepatocytes (L8824) for 24 h, and then the L8824 were incubated with sodium selenite in presence or absence of an autophagy inhibitor for 24 h. L8824 was analyzed for triglyceride concentration, immunofluorescence, and gene and protein expression levels associated with autophagy. We found that dietary nano-Se improved the growth of fish fed HFD and also decreased hepatosomatic index and intraperitoneal fat ratio of fish fed HFD (P < 0.05). HFD significantly increased hepatopancreas lipid accumulation and decreased autophagic activity (P < 0.05). Treatment of grass carp fed HFD with nano-Se decreased lipid accumulation and restored hepatic autophagy (P < 0.05). In vitro, Se (100 μM sodium selenite) obviously activated autophagy in L8824 incubated with OA, and consequently reduced the lipid accumulation induced by OA (P < 0.05). Furthermore, using pharmacological inhibition (chloroquine) of the autophagy greatly diminished the beneficial effects of Se on alleviating OA-induced lipid accumulation and increased the co-localization of lipid droplets with autophagosome (P < 0.05), which indicated that Se increased autophagic flux. In conclusion, these results suggest that Se alleviates HFD-induced hepatopancreas lipid accumulation by activating lipophagy.

相似文章

Mannan oligosaccharides alleviate oxidative injury in the head kidney and spleen in grass carp (Ctenopharyngodon idella) via the Nrf2 signaling pathway after Aeromonas hydrophila infection [Zhiyuan Lǚ, Lin Feng, Wei‐Dan Jiang, Pei Wu, Yang Liu, Jun Jiang, Sheng‐Yao Kuang, Ling Tang, Shuwei Li, Cheng-Bo Zhong, Xiao‐Qiu Zho] 畜牧与生物技术杂志(英文版) 2023,14 (1)
Selenium nanoparticles in aquaculture: Unique advantages in the production of Se-enriched grass carp (Ctenopharyngodon idella) [Chao Zhu, Zifang Wu, Qimin Liu, Xiaolin Wang, Lijuan Zheng, Shuyang He, Fangxia Yang, Hong Ji, Wuzi Don] 动物营养（英文） 2024,16
Curcumin attenuates ochratoxin A and hypoxia co-induced liver injury in grass carp (Ctenopharyngodon idella) by dual targeting endoplasmic reticulum stress and apoptosis via reducing ROS content. [Liangqin Wu, Piao Zhao, Pei Wu, Wei‐Dan Jiang, Liu Yang, Hongmei Ren, Xiao‐Wan Jin, Xiaoqiu Zhou, Lin Fen] 畜牧与生物技术杂志(英文版) 2024,15 (1)
Growth, digestive and absorptive capacity and antioxidant status in intestine and hepatopancreas of sub-adult grass carp Ctenopharyngodonidella fed graded levels of dietary threonine [Hong Yang, Wei‐Dan Jiang, Sheng‐Yao Kuang, Kai Hu, Ling Tang, Yang Liu, Jun Jiang, Yongan Zhang, Xiao‐Qiu Zhou, Lin Fen] 畜牧与生物技术杂志(英文版) 2015,6
Dietary protein levels changed the hardness of muscle by acting on muscle fiber growth and the metabolism of collagen in sub-adult grass carp (Ctenopharyngodon idella) [Min Dong, Lu Zhang, Pei Wu, Lin Feng, Wei‐Dan Jiang, Yang Liu, Sheng‐Yao Kuang, Shuwei Li, Haifeng Mi, Ling Tang, Xiao‐Qiu Zho] 畜牧与生物技术杂志(英文版) 2022,13 (1)
Aflatoxin B1 decreased flesh flavor and inhibited muscle development in grass carp (Ctenopharyngodon idella) [Xiang-Ning He, Zhen-Zhen Zeng, Wei‐Dan Jiang, Pei Wu, Yang Liu, Sheng‐Yao Kuang, Ling Tang, Shuwei Li, Lin Feng, Xiao‐Qiu Zho] 动物营养（英文） 2024,18
The role of vitamin E in polyunsaturated fatty acid synthesis and alleviating endoplasmic reticulum stress in sub-adult grass carp (Ctenopharyngodon idella). [Ke Yao, Lin Feng, Wei‐Dan Jiang, Yang Liu, Lu Zhang, Haifeng Mi, Xiao‐Qiu Zhou, Pei W] 动物营养（英文） 2024,16
Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp (Ctenopharyngodon idella): in vitro and in vivo studies [Piao Zhao, Lin Feng, Wei‐Dan Jiang, Pei Wu, Yang Liu, Jun Ren, Xiao‐Wan Jin, Lu Zhang, Haifeng Mi, Xiao‐Qiu Zho] 畜牧与生物技术杂志(英文版) 2024,15 (1)
Assessment of black soldier fly (Hermetia illucens) larvae meal as a potential substitute for soybean meal on growth performance and flesh quality of grass carp Ctenopharyngodon idellus [Zechao Hu, Handong Li, Sha Liu, Rui Xue, Jian Sun, Hong J] 动物营养（英文） 2023,14
Improving flesh quality of grass carp (Ctenopharyngodon idellus) by completely replacing dietary soybean meal with yellow mealworm (Tenebrio molitor) [Handong Li, Rongrong Xue, Jian Sun, Hong J] 动物营养（英文） 2023,12

导出引用

计量

文章访问数: 9

HTML全文浏览量: 0

PDF下载量: 0

Selenium reduces hepatopancreas lipid accumulation of grass carp (Ctenopharyngodon idella) fed high-fat diet via lipophagy activation.

相似文章

所属期刊

动物营养(英文)

推荐期刊

动物营养(英文)

畜牧与生物技术杂志(英文版)