中国农业期刊集成服务平台

当前位置: 首页 > 文章 > A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype 农业科学学报 (英文) 2024,23 (3)

Position: Home > Articles > A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype Journal of Integrative Agriculture 2024,23 (3)

A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype

作者：

Pan, Jiawei;Song, Jia;Sharif, Rahat;Xu, Xuewen;Li, Shutong;Chen, Xuehao

单位：

Yangzhou Univ, Sch Hort & Landscape Architecture, Yangzhou 225009, Peoples R China;Inst Vegetables, Tianjin Acad Agr Sci, Tianjin 300384, Peoples R China

关键词：

cucumber;yellow-lethal cotyledons;chloroplast development;yellow stripe -like transporter

摘要：

Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis, photosynthesis mechanism and chloroplast development. Herein, we identified a spontaneous mutant, yc412 , in cultivated cucumber that exhibited yellow cotyledons. The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure, and reduced photosynthetic capacity and pigment content. Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping, we narrowed the yellow cotyledons ( yc ) locus to a 96.8 kb interval on chromosome 3. By resequencing and molecular cloning, we showed that Csyc is a potential candidate gene, which encodes a yellow stripe -like (YSL) transporter. The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412 . Compared to YZU027A (WT), the expression of Csyc was significantly downregulated in the cotyledons of yc412 . Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves, mainly by suppressing the chlorophyll content. Furthermore, a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons. Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.

相似文章

Isolation and expression analysis of the ethylene receptor gene MiETR1b in mango (Mangifera indica). [Li YunHe, Zhang Zhi, Wu QingSong] 园艺学报(英文版) 2016,2 (1)
Variation of Plant Electrophysiology in Cucumber under Different Water Status [LI Guo-chen, YU Hai-ye, MA Cheng-lin, WANG Rui] 东北农业大学学报(英文版) 2005,12 (1)
Preliminary Studies on Induced Resistance of Cucumber to Downy Mildew [Wen Jingzhi, Yang Jianhua] 东北农业大学学报(英文版) 1996,3 (2)
Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development [Sun Jian-lei, Sui Xiao-lei, Huang Hong-yu, Wang Shao-hui, Wei Yu-xia, Zhang Zhen-xian] 农业科学学报 (英文) 2014,13 (5)
The synergistic advantage of combining chloropicrin or dazomet with fosthiazate nematicide to control root-knot nematode in cucumber production [Huang Bin, Wang Qian, Guo Mei-xia, Fang Wen-sheng, Wang Xiao-ning, Wang Qiu-xia, Yan Dong-donga, Ouyang Can-bin, Li Yuan, Cao Ao-cheng] 农业科学学报 (英文) 2019,18 (9)
Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber [Liu Li-ying, Duan Liu-sheng, Zhang Jia-chang, Mi Guo-quan, Zhang Xiao-lan, Zhang Zhen-xian, Ren Hua-zhong] 农业科学学报 (英文) 2013,12 (5)
What are the differences in yield formation among two cucumber (Cucumis sativus L.) cultivars and their F₁ hybrid? [Wang Xiu-juan, Kang Meng-zhen, Fan Xing-rong, Yang Li-le, Zhang Bao-gui, Huang San-wen, De Reffye, Philippe, Wang Fei-yue] 农业科学学报 (英文) 2020,19 (7)
Blue light is more essential than red light for maintaining the activities of photosystem II and I and photosynthetic electron transport capacity in cucumber leaves [Miao Yan-xiu, Wang Xiao-zhuo, Gao Li-hong, Chen Qing-yun, Qu Mei] 农业科学学报 (英文) 2016,15 (1)
Functional analysis of the nitrogen metabolism-related gene CsGS1 in cucumber [Xin Ming, Qin Zhi-wei, Yang Jing, Zhou Xiu-yan, Wang Lei] 农业科学学报 (英文) 2021,20 (6)
Melatonin treatment induces chilling tolerance by regulating the contents of polyamine, γ-aminobutyric acid, and proline in cucumber fruit [Madebo, Miilion P., Luo Si-ming, Wang Li, Zheng Yong-hua, Jin Peng] 农业科学学报 (英文) 2021,20 (11)

PDF下载 PDF预览

导出引用

计量

文章访问数: 16

HTML全文浏览量: 0

PDF下载量: 0

A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)