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Position: Home > Articles > Analysis of Functional Group of Alkaline Phosphatase from DFRKN-1 Plant Diseases and Pests 2013,4 (1)

Analysis of Functional Group of Alkaline Phosphatase from DFRKN-1

作  者：
Zhenqiang Ma;Aiying;Bo Zhang;Kaowen;Kan Yang;Zhenhua;Mou;Mingdong Y
关键词：
alkaline phosphatase;dfrkn;histidine residue;modifier;cysteine;development and utilizatio
摘  要：
To further understand characters of alkaline phosphatase and provide reference for in-depth study of the mechanism of NFRKN-1 invading into knot nematode and its development and utilization, the effects of metal ions, organic reagents and chemical modifier on activity of alkaline phosphatase from DFRKN-1 were analyzed in the paper. The results showed that Mg2+, Ba2+ and K+ under certain concentrations activated enzyme significantly and Zn2+ could inhibit enzyme activity. Methanol, ethanol and ethylene glycol inhibited enzyme activity in similar degrees. Histidine residue and Lysine residue are essential groups of alkaline phosphatase; sulfhydryl of cysteine was not an essential group, but disulfide bond played an important role in maintaining the active conformation of alkaline phosphatase.

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