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当前位置: 首页 > 文章 > 低氧环境对高原鼠兔骨骼肌HIF-1α和VEGF表达的影响 野生动物学报 2018 (2) 296-300
Position: Home > Articles > Expression of HIF-1 α and VEGF in Skeletal Muscle of Plateau Pika( Ochotona curzoniae) in Response to Hypoxic Stress Chinese Journal of Wildlife 2018 (2) 296-300

低氧环境对高原鼠兔骨骼肌HIF-1α和VEGF表达的影响

作  者：
谢惠春;罗巧玉;陈志;乔枫
关键词：
HIF-1α;VEGF;骨骼肌;低氧应激
摘  要：
研究了在低氧应激条件下,世居高原小哺乳动物高原鼠兔(Ochotona curzoniae)和SD大鼠(Rattus norregicus)骨骼肌中HIF-1α和VEGF的蛋白质水平变化及HIF-1α和VEGF的相关性。运用双位点免疫酶测定法(ELISA)检测骨骼肌中HIF-1α和VEGF的表达水平。结果表明,在低氧暴露(6 h)条件下,高原鼠兔和SD大鼠氧化和糖酵解型肌纤维中HIF-1α和VEGF的表达水平都显著高于常氧组(P<0.05)。同时发现,HIF-1α和VEGF在高原鼠兔骨骼肌中的表达增高量要显著低于SD大鼠(P<0.05)。此外,在低氧胁迫条件下,SD大鼠(n=20)和高原鼠兔(n=15)骨骼肌的氧化型肌纤维中HIF-1α和VEGF的增高(r=0.76,P<0.01)和糖酵解型肌纤维中HIF-1α和VEGF的增高(r=0.72,P<0.01)存在线性关系。同时抑制HIF-1α的表达能够显著降低骨骼肌中VEGF的表达(P<0.05)。结论 :(1)在低氧应激条件下,对比SD大鼠,高原鼠兔骨骼肌中HIF-1α和VEGF的反应功能表现出不敏感型;(2)在低氧环境中,HIF-1α对骨骼肌中VEGF起调节作用。
译  名：
Expression of HIF-1 α and VEGF in Skeletal Muscle of Plateau Pika( Ochotona curzoniae) in Response to Hypoxic Stress
作  者：
Xie Huichun;Luo Qiaoyu;Chen Zhi;Qiao Feng;Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibet Plateau in Qinghai Province,Qinghai Normal University;
单  位：
Xie Huichun%Luo Qiaoyu%Chen Zhi%Qiao Feng%Key Laboratory of Medicinal Plant and Animal Resources of Qinghai-Tibet Plateau in Qinghai Province,Qinghai Normal University
关键词：
HIF-1 α;;VEGF;;Skeletal muscle;;Hypoxic stress
摘  要：
We investigated the levels of hypoxia-inducible factor 1-alpha( HIF-1 α) and vascular endothelial growth factor( VEGF) in skeletal muscles of plateau pika( Ochotona curzoniae), a small native plateau ochotonid lagomorph, in response to hypoxia. We compared these data with those for SD rats( Rattus norvegicus). We analyzed the relationship between HIF-1 α and VEGF in skeletal muscle of plateau pika. Levels of HIF-1 α and VEGF were estimated by use of ELISA methods.Results demonstrated that hypoxic stress induced by exposure to reduced levels of O2 for 6 h significantly increased the levels of HIF-1 α and VEGF in the oxidative and glycolytic muscles of SD rats and pikas( P < 0. 05 vs. normoxic conditions). Notably, the increases in levels of HIF-1 α and VEGF were significantly less pronounced in pikas( P < 0. 05, vs. SD controls) than in SD rats.We documented a linear relationship between amplified HIF-1 α and VEGF in oxidative muscle( r =0. 76 and P < 0. 01) and glycolytic muscle( r = 0. 72 and P < 0. 01). Inhibition of HIF-1 α significantly reduced expression of VEGF( P < 0. 05). Overall, our findings suggest that:( 1) responsiveness of HIF-1 α and VEGF in skeletal muscles to hypoxic stress is blunted in plateau animals; and( 2)HIF-1 α has a regulatory effect on VEGF under hypoxic environment.

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