中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 三黄种鸡场孵化死胚中沙门菌的分离鉴定与耐药性分析 畜牧兽医学报 2018 (5) 1081-1088
Position: Home > Articles > Isolation,Identification and Antimicrobial Resistance of Salmonellafrom Dead Chicken Embryos in Sanhuang Breeder Farms Chinese Journal of Animal and Veterinary Sciences 2018 (5) 1081-1088

三黄种鸡场孵化死胚中沙门菌的分离鉴定与耐药性分析

作  者：
徐耀辉;邓同炜;齐亚如;韩文龙;卢建洲;蒋增海
关键词：
三黄鸡;死胚;沙门菌;耐药性;耐药基因
摘  要：
为了解死亡鸡胚中沙门菌的感染状况,从河南省4家三黄种鸡场自带孵化场采集死胚样品,进行沙门菌分离、血清型鉴定,并用纸片法和PCR方法对菌株耐药表型及相关耐药基因进行检测。结果:从356份死胚样品共分离到173株沙门菌,包括5种血清型:鸡白痢沙门菌(162株)、布利丹沙门菌(5株)、塞罗沙门菌(4株)、肠炎沙门菌(1株)、菲尔摩雷沙门菌(1株)。不同来源死胚中沙门菌的分离率和耐药情况差异显著;173株沙门菌对磺胺异噁唑(82.1%)、氨苄西林(74.0%)、四环素(49.1%)、土霉素(48.6%)耐药情况较严重,且43.4%(75/173)的菌株表现为多重耐药;对其他抗生素耐药情况较轻,耐药率在11%以下;对头孢他啶、美罗培南、亚胺培南、多黏菌素E、氯霉素、恩诺沙星、环丙沙星敏感。PCR共扩增到13种耐药基因,且耐药基因的检出情况与耐药表型的符合率在72%以上,其中ESBLs类、氨基糖苷类、头孢菌素类的符合率为100%。结论:三黄鸡鸡胚感染的沙门菌血清型复杂、多重耐药严重,且耐药基因普遍存在于耐药菌株中。
译  名：
Isolation,Identification and Antimicrobial Resistance of Salmonellafrom Dead Chicken Embryos in Sanhuang Breeder Farms
作  者：
XU Yao-hui;DENG Tong-wei;QI Ya-ru;HAN Wen-long;LU Jian-zhou;JIANG Zeng-hai;College of Veterinary Medicine,Henan University of Animal Husbandry and Economy;
单  位：
XU Yao-hui%DENG Tong-wei%QI Ya-ru%HAN Wen-long%LU Jian-zhou%JIANG Zeng-hai%College of Veterinary Medicine,Henan University of Animal Husbandry and Economy
关键词：
Sanhuang chicken;;dead embryo;;Salmonella;;resistance;;drug-resistant gene
摘  要：
To investigate current infection statuses of Salmonellain dead chicken embryos,Salmonellastrains was isolated and identified from dead embryo samples collected from 4 hatcheries attached to their parental flocks of Sanhuang Chickens,and the drug resistance and resistance genes were detected by Kirby-Bauer Test and PCR method,respectively.A total of 173 Salmonellaisolates were obtained from 356 dead embryos,including 5 serotypes:S.pullorum(162),S.blegdam(5),S.Cerro(4),S.enteritidis(1),S.Fillmore(1).Results showed that the isolation rate and drug resistance of Salmonellain dead embryos from different sources were significantly different.Of total 173 Salmonellaisolates,the drug resistance rate to Sulfisoxazole(SF),Ampicillin(AMP),Tetracycline(TE)and Oxytetracycline(OT)were 82.1%,74.0%,49.1% and 48.6%.And 43.4%(75/173)isolates showed multiple drug resistance.The resistance to other drugs was mild,and the resistance rate was below 11%.The isolates were sensitive to Ceftazi-dime(CAZ),Meropenem(MEM),Imipenem(IPM),Polymyxin E(CT),Chloramphenicol(C),Enrofloxacin(ENR)and Ciprofloxacin(CIP).In addition,13 drug-resistant genes were detected by PCR,and the coincidence rate between drug-resistant phenotype and drug-resistant genes was more than 72%,especially the rate were 100% to extended-spectrum beta-lactamases(ESBLs),aminoglycosides,cephalosporins.The results indicate that serotypes of Salmonella infected in Sanhuang chicken embryos are complex,the multi-drug resistance is serious and the resistance genes are widely existed in these resistant strains.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46