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马副蛔虫卵的鉴定及玻片标本的制作

作  者:
道尔加拉;苏布赛;张梦圆;樊新丽;宋瑞其;巴音查汗
关键词:
普氏野马;马副蛔虫卵;永久性标本
摘  要:
为了探索马副蛔虫虫卵玻片永久性标本制作的理想方法,提供优质的标本资源,提高实验课教学的质量,笔者收集驱虫后的普氏野马马副蛔虫(雌),借助手术刀片切开其腹腔,取出子宫,离心沉淀后收集虫卵,取适量的虫卵与甲醛中混匀,制作玻片标本.结果表明,驱虫前后马副蛔虫感染率分别为87.50%和36.84%,感染强度(平均EPG)为128.6和43.6;制作的虫卵标本能准确显示蛔虫卵的原始形态,其形态特征保持原状,能克服气泡、发霉、变形等问题,具有经济实惠、能长久保存等特点.
作  者:
DAO Erjiala;SU Busai;ZHANG Mengyuan;FAN Xinli;SONG Ruiqi;BA yinchahan;College of Animal Medicine, Xinjiang Agriculture University;Xinjiang Hejing County Animal husbandry and veterinary station;
关键词:
Equus przewalskii;;Horse Ascaris eggs;;Permanent specimen
摘  要:
To explore an ideal preparation technique of permanent slide specimens for egg of Parascaris equorum for providing the high quality samples and enhance the quality of experimental teaching.Female Parascaris equorum samples were collected after expelling parasite,dissected the Parascaris equorum and remove the uterus and collected eggs by centrifuge.Mixing the eggs and formaldehyde and then make the specimens.The result showed that before expelling parasite,the infection rate of Parascaris equorum was 87.5%,and after expelling parasite the infection rate was 36.84%,insect repellent before and after the EPG were 128.6 and 43.6,respectively.The egg specimens could display the natural form of egg of Parascaris equorum and the merphological characteristics could remain unchanged.Furthermore,the specimens can overcome many problems like bubble、mildew and deformation and have the advantages of economical benefit and long-term preservation at the same time.

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