中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同基因型烟草对大鼠脾组织凋亡相关蛋白表达的影响 山西农业科学 2018 (6) 915-919
Position: Home > Articles > Effects of Different Genotype Tobacco on the Expression of Apoptosis Related Proteins in Rat Spleen Tissue Journal of Shanxi Agricultural Sciences 2018 (6) 915-919

不同基因型烟草对大鼠脾组织凋亡相关蛋白表达的影响

作  者：
杜彬;魏克强
关键词：
烟草品种;烟气暴露;脾脏;Bcl-2;Bax
摘  要：
清洁级雄性SD大鼠随机分为正常空气对照组和2个烟气暴露处理组(T-1,T-2),全烟气暴露法染毒,分别于28,56 d取脾组织采用免疫组织化学法测定Bax和Bcl-2的表达水平,分析Bcl-2/Bax值,比较不同基因型烟草对大鼠脾组织凋亡相关蛋白表达的影响。结果表明,与对照组相比,染毒后大鼠脾白髓(WP)中Bcl-2的水平显著上升(P<0.05),Bax的水平无明显变化(P>0.05),Bcl-2/Bax值均明显升高且T-2组显著高于T-1组(P<0.05);脾红髓(RP)中Bcl-2和Bax的水平显著上升(P<0.05),但Bcl-2/Bax值均明显下降(P<0.05),且T-1与T-2组间无显著差异(P>0.05)。不同基因型烟草对大鼠脾组织凋亡相关蛋白的表达存在明显差异,这可能与不同基因型烟草化学成分不同有关,Bcl-2/Bax值有可能作为评价烟草体内毒理学效应的潜在指标。
译  名：
Effects of Different Genotype Tobacco on the Expression of Apoptosis Related Proteins in Rat Spleen Tissue
作  者：
DU Bin;WEI Keqiang;College of Life Science,Shanxi University;
关键词：
tobacco variety;;cigarette smoke exposure;;spleen;;Bcl-2;;Bax
摘  要：
To compare the effects of different genotype tobacco on expression of apoptosis related proteins in rat spleen tissue, the expression of Bcl-2 and Bax and the ratio of Bcl-2/Bax were analyzed. Twenty-four male SD rats were divided into control group and two treatment groups(T-1 and T-2)randomly, and the experimental animals were exposed to tobacco smoke, respectively. The spleen tissues were sampled at 28 d and 56 d, and the levels of Bax and Bcl-2 were detected with immunohistochemical method, then the Bcl-2/Bax were analyzed. The result showed that compared with control group, the Bcl-2 levels in white pulp(WP)increased significantly(P<0.05), but there were no obvious change with Bax expression(P>0.05), resulting in a significant increase of Bcl-2/Bax(P<0.05), and the Bcl-2/Bax in T-2 group was significantly higher than that in T-1 group(P<0.05). Meanwhile, the levels of Bcl-2 and Bax in red pulp(RP)both increased significantly(P <0.05), but the Bcl-2/Bax decreased obviously(P <0.05), and there was no significant difference between T-1 and T-2 group(P>0.05). There were differences in the effects of different genotype tobacco on the expression of apoptosis related proteins in the spleen tissue of rats. This may be related to the differences of the chemical components between two genotype tobacco. In addition, the Bcl-2/Bax could be a potential indicator for evaluating the toxicological effects of tobacco in vivo.

相似文章

导出引用
计量
文章访问数: 13
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46