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当前位置: 首页 > 文章 > ZD制剂对RAW264.7细胞分泌TNF-α水平及TNF-α诱导L929细胞死亡的影响 畜牧与饲料科学 2018 (5) 7-10
Position: Home > Articles > Effects of ZD Preparation on Secretion of TNF-α by RAW264.7 Cells and TNF-αInduced L929 Cells Death Animal Husbandry and Feed Science 2018 (5) 7-10

ZD制剂对RAW264.7细胞分泌TNF-α水平及TNF-α诱导L929细胞死亡的影响

作  者：
蒋瑞东;高鑫;李云章
关键词：
ZD制剂;TNF-α;小鼠腹腔巨噬细胞RAW264.7细胞系;小鼠成纤维细胞L929
摘  要：
[目的]研究ZD制剂对炎症模型细胞中TNF-α含量及对TNF-α诱导小鼠成纤维细胞L929死亡的影响。[方法]通过脂多糖(LPS)刺激小鼠腹腔巨噬细胞RAW264.7细胞系来构建炎症细胞模型,使用5种不同浓度(100、10、1、0.1、0.01μg/m L)的ZD制剂进行试验,并用ELISA法检测ZD制剂干预前后细胞上清液中TNF-α浓度。通过TNF-α刺激小鼠成纤维细胞L929细胞系来构建细胞毒性模型,采用MTT法检测ZD制剂干预前后L929细胞活力。[结果]ZD制剂浓度为100、10、1、0.1μg/m L时,TNF-α浓度与LPS模型组相比,差异极显著(P<0.01);ZD制剂浓度为0.01μg/m L时,TNF-α浓度与LPS模型组相比,差异显著(P<0.05)。经100、10μg/m L ZD制剂干预后的小鼠成纤维细胞L929细胞活力与TNF-α组相比显著提高。[结论]ZD制剂可以通过降低TNF-α的分泌量和降低TNF-α的生物学活性来达到抗炎的目的。
译  名：
Effects of ZD Preparation on Secretion of TNF-α by RAW264.7 Cells and TNF-αInduced L929 Cells Death
作  者：
JIANG Rui-dong;GAO Xin;LI Yun-zhang;College of Veterinary Medicine,Inner Mongolia Agricultural University;
关键词：
ZD preparation;;TNF-α;;mouse peritoneal macrophage RAW264.7 cell line;;mouse fibroblast L929 cells
摘  要：
[Objective]To study the effects of ZD preparation on the concentration of TNF-α in a cellular model of inflammation and the death of mouse fibroblast L929 cells induced by TNF-α. [Methods] The mouse peritoneal macrophage RAW264.7 cell line was stimulated by lipopolysaccharide(LPS) to establish a cellular model of inflammation, and five different concentrations of ZD preparation(100, 10, 1, 0.1, 0.01 μg/m L) were included in this study. ELISA method was used to detect the TNF-αconcentration in the cell supernatant before and after ZD preparation intervention. The cytotoxic model was established with mouse fibroblast L929 cell line by stimulation of TNF-α, and the activity of L929 cells was determined by MTT method before and after the intervention of ZD preparation. [Results] The 100, 10, 1 and 0.1 μg/m L of ZD preparation groups had extremely significant difference in TNF-α concentration compared to the LPS model group(P<0.01), and the 0.01 μg/m L of ZD preparation group showed significant difference in TNF-α concentration compared to the LPS model group(P<0.05). In addition, the activity of mouse fibroblast L929 cells were significantly elevated after the intervention of 100 and 10 μg/m L of ZD preparation compared to the TNF-α group. [Conclusion] ZD preparation exerts the anti-inflammatory effects via reducing the secretion of TNF-α and inhibiting the biological activity of TNF-α.

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