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当前位置: 首页 > 文章 > 大肠杆菌热稳定肠毒素Ⅰ和卵清蛋白融合蛋白的表达及免疫原性分析 河南农业科学 2018 (4) 141-144
Position: Home > Articles > Expression and Immunogenicity Analysis of 3 × STa-ovalbumin Fusion Protein Journal of Henan Agricultural Sciences 2018 (4) 141-144

大肠杆菌热稳定肠毒素Ⅰ和卵清蛋白融合蛋白的表达及免疫原性分析

作  者：
王录军;段强德;冯丽丽
关键词：
产肠毒素性大肠杆菌;STa毒素;融合蛋白;免疫原性;疫苗
摘  要：
为探究大肠杆菌热稳定肠毒素Ⅰ(Heat-stable toxin typeⅠ,STa)和鸡卵清蛋白(Chicken ovalbumin)串联表达的3×STa-ovalbumin重组融合蛋白的免疫原性,原核表达融合蛋白后,经SDS-PAGE和Western blot方法鉴定,将表达的融合蛋白纯化后免疫小鼠,利用ELISA方法测定免疫小鼠血清中抗STa抗体的滴度,应用体外中和试验检测抗体对STa毒素的中和作用。SDSPAGE检测结果显示,融合蛋白分子质量约42 ku;Western blot分析结果显示,融合蛋白可被STa阳性血清特异性识别。小鼠免疫试验结果表明,融合蛋白具有较强的免疫原性,诱导机体产生的抗STa抗体滴度达到2.33,并且产生的抗体能在体外中和STa的毒性作用。以上结果表明,3×STaovalbumin融合蛋白具有良好的免疫原性。
译  名：
Expression and Immunogenicity Analysis of 3 × STa-ovalbumin Fusion Protein
作  者：
WANG Lujun;DUAN Qiangde;FENG Lili;Weinan Vocational and Technical College;Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences;
单  位：
WANG Lujun%DUAN Qiangde%FENG Lili%Weinan Vocational and Technical College%Institute of Agricultural Economics and Information,Henan Academy of Agricultural Sciences
关键词：
ETEC;;STa toxin;;Fusion protein;;Immunogenicity;;Vaccine
摘  要：
To evaluate the immunogenicity of 3 × STa-ovalbumin fusion protein,the 3 × STa-ovalbumin was expressed in E. coli BL21 and verified by SDS-PAGE and Western blot. The titers of anti-STa antibody ofserum from the mice immunized with 3 × STa-ovalbumin fusion protein were detected by ELISA,and the neutralization activity of anti-STa antibody was tested by STa neutralization assay. SDS-PAGE result showed that the fusion protein was proved to be approximately 42 ku of molecular mass. Western blot assayindicated that the fusion protein could be specifically recognized by anti-STa serum. Furthermore,anti-STa serum titers were detected at 2. 33 from the immunized mice,and the anti-STa antibodies possessed neutralization activity in vitro. The results indicated the expressed fusion protein had a good immunogenicity,which could be a candidate for ETEC subunit vaccine.

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