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Position: Home > Articles > Sterilization Mechanism of AEW on Three Kinds of Food Borne Pathogenic Bacteria by Using Two Dimensional Infrared Correlation Spectroscopy Journal of Shandong Agricultural University(Natural Science Edition) 2018 (3) 424-428

基于二维相关红外光谱强酸性电解水对三种食源性致病菌的灭菌机制

作  者：
林艳宁;陶宁萍;卢瑛;赵勇;许长华
关键词：
强酸性电解水;食源性致病菌;二维相关红外光谱;灭菌机制
摘  要：
本研究旨在利用二维相关红外光谱结合红外光谱差减法探究强酸性电解水(Acidic electrolyzed water,AEW)杀灭食源性致病菌的分子作用机制。经AEW处理后的三种常见食源性致病菌(副溶血性弧菌、沙门氏杆菌、单增李斯特菌),以其和电解水接触时间为微扰条件,分析其和电解水接触过程中光谱动态变化过程,获取电解水处理对其相应化学基团的干扰信息。结果显示,AEW使致病菌蛋白质二级结构发生改变,使β折叠含量逐渐增高,β转角含量逐渐降低。本研究为以后利用光谱技术揭示电解水灭菌分子机制奠定了基础。
译  名：
Sterilization Mechanism of AEW on Three Kinds of Food Borne Pathogenic Bacteria by Using Two Dimensional Infrared Correlation Spectroscopy
作  者：
LINYan-ning;TAO Ning-ping;LUYing;ZHAOYong;XU Chang-hua;College of Food Sciences & Technology/Shanghai Ocean University;Research Center of Shanghai Aquatic Products Processing and Storage Engineering Technology;Aquatic Products Storageand Preservation Quality Safety Risk Assessment Laboratoryof Agricultural Ministry;
单  位：
LINYan-ning%TAO Ning-ping%LUYing%ZHAOYong%XU Chang-hua%College of Food Sciences & Technology/Shanghai Ocean University%Research Center of Shanghai Aquatic Products Processing and Storage Engineering Technology%Aquatic Products Storageand Preservation Quality Safety Risk Assessment Laboratoryof Agricultural Ministry
关键词：
Acidic electrolyzed water(AEW);;food-borne pathogenic bacteria;;two dimensional correlation infrared spectroscopy;;sterilization mechanism
摘  要：
The purpose of this study was to explore the molecular mechanism of killing foodborne pathogenic bacteria(AEW)in strongly acidic electrolytic water(Acidic electrolyzed water) by two dimensional correlation infrared spectroscopy(IR)and infrared spectral subtraction(IR). Three common foodborne pathogenic bacteria(Vibrio parahemolyticus, Salmonella enterica and Listeria monocytogenes) treated with AEW were used as perturbation conditions to analyze the dynamic changes of spectrum during contact with electrolytic water. The interference information of electrolytic water treatment on the corresponding chemical groups was obtained. The results shown that AEW changed the secondary structure of protein,increased the content of β-fold and decreased the content of β-corner. This study laid a foundation for revealing the molecular mechanism of electrolytic water sterilization by spectral technique.

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