中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 桑黄多糖分离纯化及其结构初步鉴定 食品科学 2006,27 (12) 446-450
Position: Home > Articles > Segregation and Purification of Polysaccharides from Phellinus igniariu and Structure Appraisal of This Fraction FOOD SCIENCE 2006,27 (12) 446-450

桑黄多糖分离纯化及其结构初步鉴定

作  者：
贾建波;李相前;杨文;张恒
单  位：
淮阴工学院生物工程系
关键词：
桑黄多糖;分离;纯化;结构鉴定
摘  要：
本文用离子交换和凝胶层析对桑黄多糖进行了纯化,并对多糖的结构、组成及理化常数进行了分析。结果表明,经弱碱性阴离子和弱酸性阳离子交换树脂一次串联脱蛋白,蛋白脱除率达94.96%,多糖回收率为77.77%。再经凝胶层析,得到大分子量多糖组分HHM(2.84×106Da)和小分子量多糖组分HLM(5.33×104Da),HLM和HHM的旋光度分别为[α]D25=+64.8°和[α]D25=+58.4°,由IR分析,初步推测该HHM和HLM多糖为β型吡喃多糖,由TLC法测得的HHM的单糖组成为葡萄糖,HLM单糖组成分别为葡萄糖和半乳糖。
译  名：
Segregation and Purification of Polysaccharides from Phellinus igniariu and Structure Appraisal of This Fraction
作  者：
JIA Jian-bo1,2,LI Xiang-qian1,YANG Wen1,ZHANG Heng1 (1.Department of Biology Engineering, Huaiyin Institute of Technology, Huaian 223001, China; 2.College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing 210009, China)
关键词：
polysaccharides of Phellinus igniariu;segregation;purification;structure appraisal
摘  要：
In this dissertation, we studied anion and cation exchange and to purification of polysaccharides from Phellinus igniariu. The polysaccharide structural and some physicochemical characters of this fraction were researched. Based on experimental results and general consideration, it was suitable to adopt using anion and cation exchange resin in series once. After that, 94.96% protein of crude polysaccharides could be removed, the recovery rate of polysaccharide was 77.77%. Higher molecular weight (HHM) and lower molecular weight(HLM) were gained by gel chromatograph. The optical rotation degree of Phellinus Igniariu polysaccharides [α]D25=+64.8°, [α]D25=+58.4°. Finally we study the structural of polysaccharides through IR, the results showed that the main polysaccharide chain was β-pyran-polysaccharide. Besides the monose of HLM through TLC analysis are glucose and galactose. The monose of HHM through TLC analysis are glucose.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46