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Position: Home > Articles > Callus Induction of Crocus sativus L. Journal of Anhui Agricultural Sciences 2016 (3) 123-125

藏红花愈伤组织的诱导

作  者:
陈明亮;张丽丽;秦路平;周志疆
单  位:
江苏丰收大地种业发展有限公司;第二军医大学
关键词:
藏红花;愈伤组织;植物激素;椰乳
摘  要:
[目的]建立藏红花愈伤组织诱导方法。[方法]以藏红花愈伤组织诱导率、诱导时间、外观形态作为评价参数,研究了外植体、激素种类及配比、外源添加物椰乳和活性炭浓度对藏红花愈伤组织诱导的影响。[结果]适宜的外植体是藏红花种球,适宜的激素种类及配比是MS+KT 0.5 mg/L+NAA 2.0 mg/L,适宜的椰乳浓度是100 m L/L,活性炭浓度是3 g/L。[结论]建立的藏红花愈伤组织诱导体系稳定性好,增殖速度较快,为工厂化生产奠定基础。
译  名:
Callus Induction of Crocus sativus L.
作  者:
CHEN Ming-liang;ZHANG Li-li;QIN Lu-ping;ZHOU Zhi-jiang;Jiangsu Fengshou Seed Industry Development Co.,Ltd.;The Second Military Medical University;
关键词:
Crocus sativus L.;;Callus;;Plant hormones;;Coconut milk
摘  要:
[Objective] To establish a callus induction method for Crocus sativus L.[Method]The explant,hormone type and combination,additives( coconut milk and active carbon) concentration were optimized. The appearance,callus induction rate and induction time were taken as the evaluation parameters.[Result] The proper explant was C. sativus seedball; proper hormone type and combination was MS + 0. 5 mg / L KT +2. 0 mg / L NAA. The suitable concentrations of coconut milk and active carbon in culture medium were 100 m L / L and 3 g / L,respectively.[Conclusion]This callus induction system for C. sativus has good stability and rapid multiplication rate,which lays foundation for the industrialized production.

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