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Position: Home > Articles > Study on HPLC Determination of Milidiomycin in Fermentation Broth by Rapid Column Modern Agrochemicals 2005,4 (2) 22-23

生物发酵液中氨苷霉素的快速分离柱高效液相色谱法测定

作  者:
章新;朱惠贤;王林;吴献花;杨光宇
单  位:
玉溪师范学院化学与环境科学系
关键词:
高效液相色谱;氨苷霉素;生物发酵液
摘  要:
研究了用快速分离柱高效液相色谱法测定生物发酵液中的氨苷霉素。生物发酵液中的氨苷霉素用ZORBAX Stable Bound (4.6×50 mm,1.8 μm) C18 快速分离柱为固定相,甲醇- 0.01 mol/L的磷酸(10:90) 为流动相分离,流速为2.0 ml/min;在该色谱条件下,氨苷霉素在1.0 min内可达到基线分离;用紫外二极管矩阵检测器检测。方法标准回收率为98%~102%,相对标准偏差为0.64%~0.87%。方法用于几种生物发酵液样品中氨苷霉素的测定,取得满意结果。
译  名:
Study on HPLC Determination of Milidiomycin in Fermentation Broth by Rapid Column
作  者:
ZHANG Xin, ZHU Hui-xian, WANG Lin, WU Xian-hua, YANG Guang-yu (Department of Chemistry and environmental science, Yuxi Teacher’s College, Yunnan Yuxi 653100, China)
关键词:
high performance liquid chromatography; milidiomycin; fermentation broth
摘  要:
A rapid column high performance liquid chromatography (HPLC) method for the determination of milidiomycin in fermentation broth was studied. The milidiomycin was separated on a ZORBAX Stable Bound (4.6×50 mm, 1.8 μm) rapid chromatographic column with methanol–0.01 mol/L phosphoric acid (10:90) as mobile phase at a flow-rate of 2.0 ml/min, and monitored with the photodiode array detector at 254 nm. The recoveries of the milidiomycin are 98%~102%, and the relative standard deviations are 0.64%~0.87%. This method was applied to the determination of milidiomycin in fermentation broth with good results.

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