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当前位置: 首页 > 文章 > 低温胁迫对不同早熟禾品种糖酵解代谢及其相关基因表达的影响 草地学报 2019,27 (6) 1503-1510
Position: Home > Articles > Effects of Low-temperature Stress on Glycolysis Metabolism and Related Gene Expression of Different Poa pratensis Varieties Acta Agrestia Sinica 2019,27 (6) 1503-1510

低温胁迫对不同早熟禾品种糖酵解代谢及其相关基因表达的影响

作  者：
董文科;马祥;张玉娟;郭睿;张兆恒;张岩;马晖玲
单  位：
青藏高原优良牧草种质资源利用重点实验室青海省畜牧兽医科学院;甘肃农业大学草业学院草业生态系统教育部重点实验室甘肃省草业工程实验室中-美草地畜牧业可持续发展研究中心
关键词：
低温胁迫;青海扁茎早熟禾;糖酵解;基因表达
摘  要：
为了探究青海扁茎早熟禾(Poa pratensis var. anceps cv. Qinghai,PQ)糖酵解代谢与其耐寒性的关系,以对温度敏感的草地早熟禾‘巴润’(Poa pratensis′ Baron′,PB)为对照,在人工气候培养箱中模拟低温(0℃)胁迫,并测定2种材料的细胞膜伤害情况及与糖酵解途径相关的指标。结果表明:低温胁迫下,PQ的细胞膜损伤程度显著低于PB,表现出较强的抗寒性;同时,低温胁迫可引起PQ中可溶性糖、蔗糖、果糖和丙酮酸含量的上升,且显著高于PB;低温胁迫下,PQ的己糖激酶(Hexokinase,HxK)、磷酸果糖激酶(Phosphofructokinase,PFK)和丙酮酸激酶(Pyruvatekinase,PK)活性均显著提高,其中HxK和PFK对低温反应更敏感;PQ中上述3种关键酶对应编码的PpHxK、PpPFK和PpPK基因的相对表达量在低温胁迫下也明显被诱导上调,且上调幅度均高于PB。以上结果表明,PQ在低温胁迫下可以有效调控糖酵解代谢,进而促进糖类及丙酮酸的积累与分解,以缓解低温胁迫对其造成的伤害。
译  名：
Effects of Low-temperature Stress on Glycolysis Metabolism and Related Gene Expression of Different Poa pratensis Varieties
作  者：
DONG Wen-ke;MA Xiang;ZHANG Yu-juan;GUO Rui;ZHANG Zhao-heng;ZHANG Yan;MA Hui-ling;College of Pratacultural Science,Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province, Sino-U.S. Center for Grassland Ecosystem Sustainability;Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine;
关键词：
Low temperature stress;;Poa pratensis var. anceps cv. Qinghai;;Glycolysis;;Gene expression
摘  要：
In order to explore the relationship between glycolysis metabolism of Poa pratensis var. anceps cv. Qinghai(PQ) and cold resistance,we used Poa pratensis ′Baron′(PB) as a control and placed the two materials in an artificial climate incubator at 0 ℃ to simulate low temperature stress. At the same time,cell membrane damage and physiological indexes related to glycolysis pathway of the two materials were determined. The results showed that the damage degree of cell membrane of PQ was significantly lower than that of PB under low temperature stress,showing strong cold resistance. Moreover,low temperature stress could cause the increase of soluble sugar,sucrose,fructose and pyruvic acid content in PQ,and was significantly higher than that of PB. The activities of hexokinase,phosphofructokinase and pyruvatekinase of PQ were also significantly increased under low temperature stress. Hexokinase and phosphofructokinase were more sensitive to low temperature and increased rapidly at the early stage of stress. Similarly,the relative expression levels of PpHxK,PpPFK and PpPK genes were significantly increased after low temperature stress,and the up-regulation amplitude was higher than that of PB. In conclusion,PQ can effectively regulate glycolysis metabolism under low temperature stress,and then promote the accumulation and decomposition of carbohydrates and pyruvate acid to alleviate the damage caused by low temperature stress.

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