中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 双芽巴贝斯虫TaqMan实时荧光PCR检测方法的建立 中国兽医学报 2014,34 (5) 747-750
Position: Home > Articles > Establishment of a TaqMan real-time PCR for detection of Babesia bigemina Chinese Journal of Veterinary Science 2014,34 (5) 747-750

双芽巴贝斯虫TaqMan实时荧光PCR检测方法的建立

作  者：
刘启生;王振宝;曹雯丽;杜晓杰;哈森;刘绪斌;巴音查汗
单  位：
伊犁出入境检验检疫局;新疆农业大学动物医学学院
关键词：
双芽巴贝斯虫;实时荧光PCR;检测;TaqMan探针
摘  要：
根据双芽巴贝斯虫(Babesia bigemina)18SrRNA基因的保守序列,设计特异性引物和TaqMan探针,建立了检测双芽巴贝斯虫的实时荧光PCR方法。该方法灵敏度高,最小检出量为1.1×101 copies/μL的标准质粒,比常规PCR灵敏1 000倍;重复性好,组内和组间重复性试验的变异系数分别为0.21%~1.14%和0.31%~1.50%,均小于2%;特异性强,对牛常见的其他两种血液原虫和健康血液无交叉反应。用建立的实时荧光PCR和常规PCR分别对30份临床样品进行检测,实时荧光PCR和常规PCR的检出率分别为30%和16.7%。结果表明,本研究建立的荧光TaqMan实时荧光PCR方法可以灵敏、准确、快速检测双芽巴贝斯虫感染,将为蜱传牛梨形虫病的流行病学调查和防制提供新的方法。
译  名：
Establishment of a TaqMan real-time PCR for detection of Babesia bigemina
作  者：
LIU Qi-sheng;WANG Zhen-bao;CAO Wen-li;DU Xiao-jie;HA Sen;LIU Xu-bin;Bayinchahan;College of Animal Medicine,Xinjiang Agriculture University;Yili Entry-exit Inspection and Quarantine Bureau;
关键词：
Babesia bigemina;;real-time PCR;;TaqMan probe;;detection
摘  要：
A pair of primers and a TaqMan probe was designed according to the conserved sequence of the 18SrRNA gene of B.bigemina,and a TaqMan real-time PCR method was established for detection of B.bigemina.The sensitivity of the method was 1.1×101 copies/μL which was 1 000 times more than conventional PCR.And the reproducibility tests in inter-assay and intra-assay indicated that the coefficients of variation were 0.21%-1.14%and 0.31%-1.50%,respectively,less than 2%.Moreover,the method was also highly specificity and had no cross-reaction with other bovine piroplasms.A total of 30clinical samples were tested by the established real-time PCR and the conventional PCR,respectively,the positive rates by the real-time PCR was 30%,but 16.7% positive by the conventional PCR.The real-time PCR method for detection of B.bigemina was sensitive,specific and rapid detection technology,which will provided a strong guarantee for the tick-borne bovine piroplasmosis control and epidemiological investigation.

相似文章

导出引用
计量
文章访问数: 1
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46