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当前位置: 首页 > 文章 > 甘肃棘豆中降解苦马豆素内生细菌的筛选与鉴定 浙江大学学报(农业与生命科学版) 2011,37 (5) 521-526
Position: Home > Articles > Screening and identification of endophytic bacteria of Oxytropis kansuensis Bunge for swainsonine degradation Journal of Zhejiang University (Agriculture and Life Sciences) 2011,37 (5) 521-526

甘肃棘豆中降解苦马豆素内生细菌的筛选与鉴定

作  者：
胡延春;刘鹏;赵春蕊;郑传廷;刘玲
单  位：
四川农业大学动物医学院临床兽医学系
关键词：
甘肃棘豆;内生细菌;苦马豆素;生物降解;缺陷短波单胞菌
摘  要：
为研究甘肃棘豆(Oxytropis kansuensis Bunge)中降解疯草毒素——苦马豆素的内生细菌,采用选择性富集培养的方法,以苦马豆素作为唯一碳源,从甘肃棘豆内生细菌中筛选到1株苦马豆素降解菌,命名为Ab.在摇床转速为150 r·min-1、28℃条件下培养30 h,菌株Ab对质量浓度为100 mg·L-1苦马豆素的降解率为11.577 1%;延长培养时间并不能提高其降解苦马豆素的能力,但可耐受高质量浓度(1 g·L-1)的苦马豆素.通过形态学、生理生化试验和16S rDNA序列比对分析对Ab进行鉴定,结果表明,菌株Ab为革兰阴性短小杆菌,在LB平板上菌落呈圆形,中心凸起,边缘整齐,乳白色,表面光滑,属于短波单胞杆菌属(Brevundi monas),与缺陷短波单胞菌(Brevundi monas diminuta)同源性为100%.说明甘肃棘豆中存在降解苦马豆素的内生细菌Brevundi monas diminutaAb;降解苦马豆素的内生细菌可能与产生苦马豆素的内生真菌共同维持疯草中苦马豆素的动态平衡.
译  名：
Screening and identification of endophytic bacteria of Oxytropis kansuensis Bunge for swainsonine degradation
作  者：
HU Yan-chun1,2,LIU Peng1,ZHAO Chun-rui1,ZHENG Chuan-ting1,LIU Ling1 (1.Department of Clinical Veterinary Medicine,College of Veterinary Medicine,Sichuan Agricultural University,Ya'an,Sichuan 625014,China;2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural University,Ya'an,Sichuan 625014,China)
关键词：
Oxytropis kansuensis Bunge;endophytic bacteria;swainsonine;biodegradation;Brevundimonas diminuta strain Ab
摘  要：
One strain,named as Ab,which was isolated from Oxytropis kansuensis Bunge and could degrade locoweed toxin—swainsonine,was screened by selective enrichment culture when swainsonine was used as a sole carbon source.Its degradation rate could reach 11.577 1% under the circumstance of being incubated on a shaker at 150 r·min-1 and temperature at 28 ℃ within 30 hours when the concentration of swainsonine was 100 mg·L-1.The capability of degrading swainsonine could not be improved by extending cultivation time,while it could tolerate high concentration swainsonine up to 1 g·L-1.Strain Ab was identified by 16S rDNA sequencing,morphological,biochemical and physiological experiments.The results showed that strain Ab was gram-negative bacteria whose colonies were round,milk white,glossy and had raised-center and regular edge on the Luria-Bertani plate.And the colony was Brevundimonas and shared the highest homology(100%) with Brevundimonas diminuta by Blast software analysis.It is concluded that one strain of endophytic bacterium for degrading swainsonine,B.diminuta strain Ab,was existed in O.kansuensis.Therefore,it is speculated that endophytic bacteria of degrading swainsonine might keep the homeostasis of swainsonine together with endophytic fungi of producing swainsonine in locoweeds.

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