当前位置: 首页 > 文章 > 加酶提高发酵豆粕蛋白质水解度的研究 安徽农业科学 2010,38 (10) 5141-5142+5231
Position: Home > Articles > Optimization of the enzymatic hydrolysis condition to improve the degree of hydrolysis of fermented soybean meal Journal of Anhui Agricultural Sciences 2010,38 (10) 5141-5142+5231

加酶提高发酵豆粕蛋白质水解度的研究

作  者:
刘唤明;薛晓宁
单  位:
广东海洋大学工程学院;广东海洋大学食品科技学院
关键词:
发酵豆粕;水解度;混菌固态发酵
摘  要:
[目的]在枯草芽孢杆菌、乳酸菌、酵母菌这3种菌混合发酵生产发酵豆粕的基础上,通过添加酶来提高发酵豆粕的水解度。[方法]通过添加中性蛋白酶、酸性蛋白酶和纤维素酶进行单因子试验和正交试验优化。[结果]结果表明,最佳加酶条件为中性蛋白酶加酶量为20U/g,酸性蛋白酶加酶量为20U/g,纤维素酶加酶量为6U/g;并且在此加酶优化条件下,发酵豆粕的水解度由优化前的9.32%增加到19.65%。[结论]添加中性蛋白酶、酸性蛋白酶和纤维素酶能显著提高发酵豆粕蛋白质水解度。
译  名:
Optimization of the enzymatic hydrolysis condition to improve the degree of hydrolysis of fermented soybean meal
作  者:
LIU Huan-ming et al (School of Food Science and Technology of Guang Dong Ocean University,ZhanJiang,Guangdong 524088 )
关键词:
Fermented soybean meal;Degree of hydrolysis;Multi-strain solid fermentation
摘  要:
[Objective] Enzyme was used to increase the degree of hydrolysis of fermented soybean meal produced by multi-strain solid fermentation with Bacillus subtilis,Yeast and Lactic acid bacteria.[Method] Single-factor experiments and orthodox experiments were conducted by adding neutral proteinase,acidic proteinase and cellulase.[Result] The results showed that the optimum enzymatic hydrolysis conditions were the neutral proteinase concentration of 20 U/g,the acidic proteinase concentration of 20 U/g,the cellulase concentration of 6 U/g.And under the above conditions,the degree of hydrolysis of soybean protein increased from 9.32% to 19.65.[Conclusion] The degree of hydrolysis of soybean protein increased markedly by adding neutral proteinase,acidic proteinase and cellulase.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊