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当前位置: 首页 > 文章 > 金针菇耐高温新菌株的诱变选育 中国食用菌 2016 (4) 18-23
Position: Home > Articles > A Novel High-temperature-tolerant Strain of Flammulina velutipes by Mutagenesis Edible Fungi of China 2016 (4) 18-23

金针菇耐高温新菌株的诱变选育

作  者：
罗润;郭丽琼;林俊芳;韩飞;李琼洁;康林芝
单  位：
广东汕头质量与计量监督检测研究所;华南农业大学食品学院华南农业大学食品生物技术研究所;广东省微生态制剂工程技术研究中心
关键词：
金针菇;诱变育种;诱变剂;高温品种
摘  要：
以黄色金针菇(Flammulina velutipes)菌株FV7为材料,采用紫外线诱变、氯化锂诱变和甲基磺酸乙酯(EMS)诱变等多种诱变方法对金针菇的菌丝细胞进行诱变,诱变后的菌丝细胞经过菌丝体生长阶段33℃高温初筛和子实体发育阶段20℃中温复筛,并经过多轮筛选获得耐高温突变菌株FV7EMS。研究结果表明,采用甲基磺酸乙酯对金针菇菌丝细胞的诱变效果明显,突变菌株FV7EMS能在20℃温度出菇,且生长速度快、菌柄较长,生物学效率比亲本FV7提高了28.82%,与亲本间存在较大的遗传差异。金针菇耐高温新品种的培育,对于降低工厂化栽培能耗、多季节栽培金针菇有重要的科学研究价值。
译  名：
A Novel High-temperature-tolerant Strain of Flammulina velutipes by Mutagenesis
作  者：
LUO Run;GUO Li-qiong;LIN Jun-fang;HAN Fei;LI Qiong-jie;KANG Lin-zhi;College of Food Science,Institute of Food Biotechnology,South China Agricultural University;Microecologics Engineering Center of Guangdong Province;Guangdong Shantou Supervision Testing Institute of Quality and Metrology;
关键词：
Flammulina velutipes;;mutation breeding;;mutagen;;high-temperature-resistant varieties
摘  要：
The mycelial fragments of Flammulina velutipes(strain FV7, yellow) were treated with ultraviolet light(UV), ethylmethane sulfonate(EMS) and lithium chloride(LiCl). Then the mycelial cell treated with mutation was screened at 33°C during mycelial growth period and at 20°C during fruiting body development period, and heat resistant mutant strain FV7 EMS was obtained. The results showed that EMS had significant mutagenic effects on hypha cells, the mutant strain was high-temperature resistant varieties with long stipe, and it grew fast, compared with original strain FV7, the biological efficiency was higher with28.82%, which presented apparent genetic differences to the original strain. The breeding of F. velutipes with high-temperature resistance was of great importance in factory energy consumption and its multi-season cultivation.

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