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Position: Home > Articles > Observation of Mast Cells in JianBai Xiang Pig Guizhou Journal of Animal Husbandry & Veterinary Medicine 2014,38 (3) 4-7

剑白香猪各组织中肥大细胞的观察研究

作  者:
陈伟;刘忠伟;田兴贵;张依裕;刘培琼
单  位:
贵州大学生命科学学院;贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点实验室;贵州大学动物科学学院
关键词:
剑白香猪;肥大细胞;Carnoy’s固定液
摘  要:
目的:试验旨在通过对6月龄剑白香猪肥大细胞的观察,以期了解肥大细胞在剑白香猪不同组织器官的数量、分布及形态。方法:采集剑白香猪心、肝、脾、肺、肾、肾上腺、小肠等部位样品用多聚甲醛和carnoy’s液固定后,用甲苯胺蓝染色法检测各组织中肥大细胞数量、分布及形态,并用SPSS13.0软件进行统计分析。结果:试验表明,肥大细胞数量在不同性别间无明显差异;Carnoy’s固定液是测定猪各组织器官MC的较理想固定液;肥大细胞在各种组织中分布及形态复杂多样。本试验可为剑白香猪肥大细胞的进一步研究提供参考数据。
译  名:
Observation of Mast Cells in JianBai Xiang Pig
作  者:
Chen Wei;Liu Zhongwei;Tian Xinggui;Zhang Yiyu;Liu Peiqiong;Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Guizhou University;College of Animal Sciences,Guizhou University;College of Life Sciences,Guizhou University;
关键词:
Mast Cell;;JianBai Xiang Pig;;Carnoy's Fluid
摘  要:
Objective:This paper is detected and analyzed of the mast cells in different tissue of JianBai Xiang Pigs at 6 months age. Method:Collecting tissue samples of JianBai Xiang pig such as heart,liver,spleen,lung,kidney,adrenal gland and small intestine with paraformaldehyde and carnoy's liquid fixation,using toluidine blue staining method to detect the number of mast cells,distribution and morphology in the tissues. and statistical analysis was performed using SPSS13. 0 software. Result:The results showed that Mast cell number no significant difference between in different gender; The Carnoy's fluid was the best fixative in observation of MC of pigs; The distribution of mast cells in various tissues are complicated. This study provide a reference for the mini- pigs that were used the biology,medical science research.

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